- Nebraska Prostate Cancer Research Program (NPCRP)
- Core Facilities
- Presentations
- Safety and Equipment
- Instructions for use of Departmental Equipment
- ACGT Sequencing Instructions for Use
- Bacterial Shakers
- Campus Wide Cold Room Guidelines
- Developer Instructions
- BioRad Gel Doc System Instructions and Safety
- Micro Balance
- MilliQ Water Filtration System
- PCR Machine Instructions and Safety
- Microplate Reader, Molecular Devices, Spectra Max
- Speed Vac/Gel Dryer Instructions and Safety
- Ice Machine
- Dry Ice
- Cold Room
- Rotors
- Centrifuge
- Standard Bioruptor from Diagenode
- UV Light Box
- Equipment Maintenance
PCR Machine Instructions and Safety
DRC 1 7022
No current caretaker, contact Jeanette Gardner, Main office with problems.
Do not use any tube or plate that is not appropriate for the PCR machine you are using.
- Make sure tubes and especially plates are well sealed before you begin run.
- Clean up any spilled solutions and dispose of in appropriate biohazard boxes.
- Be careful with PCR machine lids. These can be damaged if you slam or drop lids.
- Turn PCR machine off when you are done using.
- Make sure PCR heater block is clean before you start a run. Check each tube receptacle before you start.
- Distribute tubes evenly across block so lid will seat flat against top of tubes for even heating and sealing.
Updated 5-10-2015
- Nebraska Prostate Cancer Research Program (NPCRP)
- Core Facilities
- Presentations
- Safety and Equipment
- Instructions for use of Departmental Equipment
- ACGT Sequencing Instructions for Use
- Bacterial Shakers
- Campus Wide Cold Room Guidelines
- Developer Instructions
- BioRad Gel Doc System Instructions and Safety
- Micro Balance
- MilliQ Water Filtration System
- PCR Machine Instructions and Safety
- Microplate Reader, Molecular Devices, Spectra Max
- Speed Vac/Gel Dryer Instructions and Safety
- Ice Machine
- Dry Ice
- Cold Room
- Rotors
- Centrifuge
- Standard Bioruptor from Diagenode
- UV Light Box
- Equipment Maintenance