The Flow Cytometry Research Facility

Flow Cytometry Research FacilityCraig L. Semerad, PhD, Director of the Flow Cytometry Research Facility (FCRF) graciously answered our questions about FCRF’s utility for CHRI members’ research efforts. 

What kind of services can you provide CHRI investigators? 
The Flow Cytometry Research Facility (FCRF) is a fee-for-services shared resource laboratory providing comprehensive research flow Cytometry services, education and training to all investigators on the UNMC campus, as well as other regional academic and corporate partners. The FCRF provides support in all areas of established protocols and development of new applications guided by investigator needs. Routine analyses include immunophenotyping, DNA and cell cycle determinations, fluorescent protein expression, bead-based assays for secreted proteins and high-speed cell sorting (bulk and single-cell sorting) using state-of-the-art instrumentation. Given the complexity, high-costs and specialized knowledge needed to operate flow cytometry instrumentation, the FCRF acts as an essential research partner to investigators in the experimental design, sample preparation, acquisition, analysis and presentation of flow cytometry data.

If an investigator wants to learn more about utilizing the services of the Flow Cytometry Research Facility, what is a good way of getting needed information?
Investigators interested in learning more about the FCRF may visit our website.
In addition, feel free to contact FCRF personnel with any questions about available services and procedures via phone or email:

Victoria Smith, B.S., FCRF Coordinator
402-559-6267
Email

Holly Britton, M.S., FCRF Technician
402-559-6267
Email

Craig Semerad, Ph.D., SCYM(ASCP)CM, FCRF Director
402-559-6299
Email
 
What are the first steps towards engaging the services of the Flow Cytometry Research Facility? 
To begin use of FCRF resources, we highly encourage new users to set up a consultation with one of the FCRF personnel to establish the scope of a user’s experiment, determine which FCRF instrumentation and resources are necessary to achieve experimental objectives and ascertain whether training is needed/wanted for continued use of the FCRF.
  
What are some common questions you get about the Flow Cytometry Research Facility and what are the answers to those questions? 

How do I get started?  
Contact the FCRF to set up a consultation with a FCRF staff member for discussion of experimental objectives and determine future use of FCRF instrumentation and resources.

Where is the Flow Lab located? 
The Flow Cytometry Research Facility is in the Durham Research Center (DRC) Room 1056 on the UNMC campus

What instrumentation is available?  
See our Equipment page on our website or contact FCRF personnel.

What are the rates for FCRF services?
See our Services and Rates page on our website or contact FCRF personnel.

Will FCRF personnel process and acquire my samples? 
The FCRF does not process and stain experimental samples. However, the FCRF offers assisted and unassisted acquisition of samples and high-speed cell sorting.

Does the FCRF provide education and training for use of facility resources? 
The FCRF holds an Introduction to Flow Cytometry class the first week of each month (mandatory for new users of the FCRF). In addition, the FCRF provides training for unassisted use of FCRF instrumentation and resources (high-speed cell sorting must be performed by FCRF personnel).  

What is your key takeaway for readers of the CHRI Research Bulletin about Flow Cytometry Research Facility?
We hope readers realize the FCRF provides access to state-of-the-art flow cytometry instrumentation and services along with the necessary expertise, support and education to enhance collaborative development and bring about positive experimental outcomes.