Pankaj Singh Publications

Selected Publications:

Shukla SK, Purohit V, Mehla K, Gunda V, Chaika NV, Vernucci E, King RJ, Abrego J, Goode GD, Dasgupta A, Illies AL, Gebregiworgis T, Dai B, Augustine JJ, Murthy D, Attri KS, Mashadova O, Grandgenett PM,, Powers R, Ly QP, Lazenby AJ, Grem J, Yu F, Matés JM, Asara JM, Kim J-W, Hankins JH, Weekes C, Hollingsworth MA, Sarcova NJ, Sasson AR, Fleming JB, Oliveto J, Lyssiotis CA, Cantley LC, Berim L, and Singh PK. MUC1 and HIF-1alpha Signaling Crosstalk Induces Anabolic Glucose Metabolism to Impart Gemcitabine Resistance to Pancreatic Cancer. Manuscript in press in Cancer Cell.

Goodwin J, Neugent ML, Lee SY, Choe JH, Choi H, Jenkins D, Ruthenborg RJ, Jeong JY, Wake M , Abe H, Takeda N, Endo N, Inoue M, Xuan  Z, Yoo H, Chen M, Ahn J-M, Minna JD, Helke KL, Singh PK, Shackelford DB, Kim J-W. Distinct Metabolic Phenotypes within Non-small Cell Lung Cancer Define Selective Vulnerability to Glycolytic Inhibition of Lung Squamous Cell Carcinoma. Manuscript in press in Nature Communications.

Abrego J, Gunda V, Vernucci E, Shukla SK, King RJ, Dasgupta A, Goode G, Murthy D, Yu F, Singh PK. GOT1-mediated anaplerotic glutamine metabolism regulates chronic acidosis stress in pancreatic cancer cells. Cancer Letters. 2017 Apr 26;400:37-46.

King RJ, Yu F, and Singh PK. Genomic alterations in mucins across cancers. Oncotarget, Advance Publications 2017.

Goode G, Gunda V, Chaika NV, Purohit V, Yu F, Singh PK. MUC1 facilitates metabolomic reprogramming in triple-negative breast cancer. PLoS One. 2017 May 2;12(5):e0176820.

Attri KS, Murthy M, Singh PK. Racial Disparity in Metabolic regulation of Cancer. 2016, Manuscript in press for Frontiers in Biosciences. 2017 Mar 1;22:1221-1246.

 Coothankandaswamy V, Cao S, Xu Y, Prasad, P, Singh PK, Reynolds CP, Yang S, Ogura J, Ganapathyu V, Bhutia Y. Amino acid transporter SLC6A14 is a novel and effective drug target for pancreatic cancer. British Journal of Pharmacology. 2016, Oct 17 Epub.

Gunda V, Yu F, Singh PK. Validation of Metabolic Alterations in Microscale Cell Culture Lysates Using Hydrophilic Interaction Liquid Chromatography (HILIC)-Tandem Mass Spectrometry-Based Metabolomics. PLoS ONE. 2016. Apr 27;11(4):e0154416.

Mondal G, Kumar V, Shukla SK, Singh PK, Mahato RI. EGFR-Targeted Polymeric Mixed Micelles Carrying Gemcitabine for Treating Pancreatic Cancer. Biomacromolecules. 2016 Jan 11;17(1):301-13.

Shonka NA, Gupta S, and Singh PK. Targeted Therapy for Glioblastoma: Lessons Learned and Future Directions. Current Cancer Therapy Reviews, 2016. 11(1): 44-58.

Shukla SK, Dasgupta A, Mehla K, Gunda V, Vernucci E, Souchek J, Goode G, King R, Mishra A, Rai I, Nagarajan S, Chaika N, Yu F, Singh PK. Silibinin-Mediated Metabolic Reprogramming Attenuates Pancreatic Cancer-Induced Cachexia And Tumor Growth. Oncotarget 2015 Dec 1;6(38):41146-61.

Yang S, Zhang L, Purohit V, Shukla S, Chen X, Yu F, Fu K, Chen Y, Solheim J, Singh PK, Song W, and Dong J. (2015) Active YAP Promotes Pancreatic Cancer Cell Motility, Invasion and Tumorigenesis in a Mitotic Phosphorylation-dependent Manner through LPAR3. Oncotarget 2015 Nov 3;6(34):36019-31

Shukla SK, Gunda V, Abrego J, Haridas D, Chaika NV, Yu F, Batra SK, and Singh PK. MUC16-mediated metabolic reprogramming induces motility and invasiveness in pancreatic cancer cells. Oncotarget. 2015 Aug 7;6(22):19118-31.

Shukla SK, Gebrgioworgis T, Purohit V, Gunda V, Radhakrishnan P, Mehla K, Pipinos II, Powers R, Yu F, Singh PK (2014). Metabolic Reprogramming Induced by Ketone Bodies Diminishes Pancreatic Cancer Cachexia. Cancer Metabolism. 2014 Sep 1;2:18.

Mehla K and Singh PK. (2014) MUC1: a novel metabolic regulator. Manuscript in press for BBA Reviews on Cancer. 2014 Apr;1845(2):126-35.

Liu X, Caffrey TC, Steele MM, Mohr A, Singh PK, Radhakrishnan P, Kelly DL, Wen Y, Hollingsworth MA (2014). MUC1 regulates cyclin D1 gene expression through p120 catenin and β-catenin. Oncogenesis. Jun 30;3:e107.

Stark JL, Mehla K, Chaika N, Acton TB, Xiao R, Singh PK, Montelione GT, and Powers R. (2014) The structure and function of human DNAJA1 and its relationship to pancreatic cancer. Biochemistry. 2014 Mar 4;53(8):1360-72.

Mehla K, Singh D, Chaudhary S, Kumar S and Singh PK (2012). Exome sequencing and advances in crop improvement. Advances in Genetics. 2012;79:87-121.

Chaika NV, Gebregiworgis T, Lewallen ME, Purohit V, Radhakrishnan P, Liu X, Zhang B, Mehla K, Brown RB, Caffrey T, Yu F, Johnson KR, Powers R, Hollingsworth MA, and Singh PK. (2012) MUC1 mucin stabilizes and activates hypoxia-inducible factor 1 alpha to regulate metabolism in pancreatic cancer. PNAS, 2012 Aug 21;109(34):13787-92. 

Singh PK, Brand RE and Mehla K. (2012) miRNAs in pancreatic cancer metabolism. Nature Reviews Gastroenterology & Hepatology 2012 Apr 17;9(6):334-44.

Chaika NV, Yu F, Purohit V, Lazenby AJ, DiMaio D, Anderson JM, Yeh JJ, Johnson KR, Hollingsworth MA and Singh PK. (2012) Differential expression of metabolic genes in tumor and stromal components in primary and metastatic loci in pancreatic adenocarcinomas. PLoS One. 2012;7(3):e32996. Epub 2012 Mar 7.

Dey P, Rachagani S, Chakraborty S, Singh PK, Zhao X, Gurumurthy CB, Anderson J, Lele S, Hollingsworth MA, Band V and Batra SK. Overexpression of Ecdysoneless (Ecd) in Pancreatic Cancer and its Role in Oncogenesis by Regulating Glycolysis. Clinical Cancer Research 2012 Nov 15;18(22):6188-98.

Torres MP, Rachagani, S, Purohit V, Pandey P, Joshi S, Moore ED, Johansson SL, Singh, PK, Ganti AK, Batra SK. (2012) Graviola: A novel promising natural-derived drug that inhibits tumorigenicity and metastasis of pancreatic cancer cells in vitro and in vivo through altering cell metabolism. Cancer Letters 2012 Oct 1;323(1):29-40.

Singh D§Singh PK§, Chaudhary S§, Mehla M§, Kumar S§. (2012). Exome sequencing and advances in crop improvement. Advances in genetics 01/2012; 79:87-121. (§ equal contribution)

Singh PK, Mehla K, Hollingsworth MA, Johnson KR. (2011) Regulation of aerobic glycolysis by miRNA in Cancer. Molecular and Cellular Pharmacology 2011;3(3):125-134..

Eggers JP, Grandgenett PM,  Collisson EA,  Behrens ME, Tremayne J, Singh PK, Swanson BJ, Anderson JM, Caffrey TC, High R, Oullette M, Hollingsworth MA. (2011). Cyclin Dependent Kinase 5 is amplified and over-expressed in pancreatic cancer and activated by mutant K-Ras. Clinical Cancer Res Oct 1 2011; 17:6140-6150; Published OnlineFirst August 8, 2011.

Song Y, Altarejos J, Goodarzi MO, Inoue H, Guo X, Berdeaux R, Kim JH, Goode J, Igata M, Paz JC, Hogan MF, Singh PK, Goebel N,Vera L, Miller N, Cui J, Jones MR; CHARGE Consortium; GIANT Consortium, Chen YD, Taylor KD, Hsueh WA, Rotter JI, Montminy M. (2010). CRTC3 links catecholamine signalling to energy balance. Nature 468(7326):933-9.

Behrens ME, Grandgenett PM, Bailey JM, Singh PK, Yi CH, Yu F, Hollingsworth MA. (2010). The reactive tumor microenvironment: MUC1 signaling directly reprograms transcription of CTGF. Oncogene 29(42):5667-77.

Singh PK,Behrens ME, Eggers JP, Cerny RL, Bailey JM, Shanmugam K, Gendler SJ, Bennett EP, Hollingsworth MA. (2008) Phosphorylation of MUC1 by Met Modulates Interaction with p53 and MMP1 Expression. J Biol Chem 283:26985-26995.

Chaturvedi P, Singh AP, Chakraborty S, Chauhan SC, Bafna S, Meza JL, Singh PK, Hollingsworth MA, Mehta PP, Batra SK. (2008) MUC4 mucin interacts with and stabilizes the HER2 oncoprotein in human pancreatic cancer cells. Cancer Res 68:2065-2070.

Bailey JM, Swanson BJ, Hamada T, Eggers JP, Singh PK, Caffery T, Ouellette MM, Hollingsworth MA. (2008) Sonic hedgehog promotes desmoplasia in pancreatic cancer. Clin Cancer Res 14:5995-6004.

Bailey JM, Singh PK, Hollingsworth MA. (2007) Cancer metastasis facilitated by developmental pathways: Sonic hedgehog, Notch, and bone morphogenic proteins. J Cell Biochem 102:829-839.

Swanson BJ, McDermott KM, Singh PK, Crocker PR, Hollingsworth, MA. (2007) MUC1 is a receptor for Myelin-Associated Glycoprotein (Siglec-4a) and their interaction contributes to adhesion in pancreatic cancer perineural invasion. Cancer Res 67:10222-10229.

Singh PK, Wen Y, Swanson BJ, Shanmugam K, Kazlauskas A, Cerny RL, Gendler SJ, Hollingsworth MA (2007) Platelet-derived growth factor receptor beta-mediated phosphorylation of MUC1 enhances invasiveness in pancreatic adenocarcinoma cells. Cancer Res 67:5201-5210.

Tsutsumida H., Swanson BJ, Singh PK, Caffrey TC, Kitajima S, Goto M, Yonezawa S, Hollingsworth MA. (2006) RNA Interference Suppression of MUC1Reduces the Growth Rate and Metastatic Phenotype of Human Pancreatic Cancer Cells. Clin Cancer Res 12:2976-2987.

Singh PK, Hollingsworth MA. (2006) Cell surface-associated mucins in signal transduction. Trends Cell Biol 16:467-476.