{"id":61266,"date":"2018-03-01T00:00:00","date_gmt":"2018-03-01T06:00:00","guid":{"rendered":"https:\/\/www.unmc.edu\/newsroom\/2018\/03\/01\/companion-technology-makes-easi-crispr-easier\/"},"modified":"2022-09-02T17:15:31","modified_gmt":"2022-09-02T22:15:31","slug":"companion-technology-makes-easi-crispr-easier","status":"publish","type":"post","link":"https:\/\/www.unmc.edu\/newsroom\/2018\/03\/01\/companion-technology-makes-easi-crispr-easier\/","title":{"rendered":"Companion technology makes Easi-CRISPR easier"},"content":{"rendered":"

UNMC Munroe-Meyer Institute researcher Channabasavaiah Gurumurthy, M.V.S.C., (D.V.M.), Ph.D., who last year announced a new tool that genetically alters mice for specific scientific studies, can now alter those genes directly into a fertilized egg in that mouse.<\/p>\n

The groundbreaking work was done in collaboration with Masato Ohtsuka, Ph.D., an associate professor at Tokai University in Japan and is published in the March issue of “Genome Biology.”<\/a><\/p>\n

The Easi (Efficient additions with ssDNA inserts)-CRISPR technology, when paired with the new discovery called Genome editing via Oviductal Nucleic Acids Delivery (GONAD) can make those genetic changes without the need for complex microinjections. <\/p>\n

The GONAD technology makes it easier to insert new sequences at the genomic cut sites, to correct disease mutation or replace a faulty gene, he said.<\/p>\n

The method generates mouse models containing single-base changes, kilobase-sized deletions and knock-ins. <\/p>\n

The efficiency of GONAD is comparable to that of traditional microinjection methods, but avoids the technically difficult steps of handling zygotes in ex vivo fashion, which requires a separate recipient animal for embryo transfer.<\/p>\n

“Such features make GONAD a perfect tool suitable for gene therapy applications,” Dr. Gurumurthy said.<\/p>\n

Findings for Easi-CRISPR were published last year in “Genome Biology”<\/a> and “Nature Protocols,”<\/a> and it was named “Best of 2017: Top Pick Genome Biology.”<\/a><\/p>\n

Drs. Gurumurthy and Masato said that both the Easi-CRISPR and GONAD methods already are being used in numerous labs worldwide thanks to the BioRxiv server where preprints of the articles (biorxiv1<\/a>, biorxiv2<\/a> and biorxiv3<\/a>) were published several months before their publications in peer-reviewed journals.<\/p>\n<\/div><\/div>","protected":false},"excerpt":{"rendered":"

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