TAMMY KIELIAN, PhD
Areas of interest include immunometabolism and how S. aureus biofilm-leukocyte metabolic crosstalk influences the epigenetic landscape of leukocytes to promote their anti-inflammatory attributes. We have recently determined that biofilm-associated monocytes are biased towards oxidative phosphorylation that leads to their anti-inflammatory bias and that metabolic reprogramming can augment their pro-inflammatory properties and biofilm clearance. Our recent study has identified a critical role for biofilm metabolism in influencing the anti-inflammatory properties of biofilm-associated leukocytes. This is through the action of S. aureus-derived lactate, which inhibits histone deacetylase 11 (HDAC11) activity and causes extensive epigenetic changes at the promoters of numerous host genes, including the key anti-inflammatory cytokine Il-10.
REPRESENTATIVE PUBLICATIONS (Trainees in bold text)
- Yamada, K.J. and Kielian, T. Biofilm-leukocyte cross-talk: impact on immune polarization and immunometabolism. Innate Immun., 11:280-288, 2019. PMID: 30347401
- Yamada, K.J., Heim, C.E., Xi, X., Attri, K.S., Wang, D., Zhang, W., Singh, P.K., Bronich, T.K., and Kielian, T. Monocyte metabolic reprogramming promotes pro-inflammatory activity and Staphylococcus aureus biofilm clearance. PLoS Path., 16(3):e1008354, 2020. PMID: 32142554
- Bosch, M.E.*, Bertrand, B.P.*, Heim, C.E., Chaudhari, S.S., Aldrich, A.L., Thomas, V.C., and Kielian, T. Staphylococcus aureus ATP synthase promotes biofilm persistence by influencing innate immunity. mBio, 11(5):e01581-20, *Equal contributions. PMID: 32900803
- Heim, C.E., Bosch, M.E., Yamada, K.J., Aldrich, A.L., Chaudhari, S.S., Klinkebiel, D., Gries, C.M., Alqarzaee, A.A., Li, Y., Thomas, V.C., Seto, E., Karpf, A.R., and Kielian, T. Lactate production by Staphylococcus aureus biofilm inhibits HDAC11 to reprogram the host immune response during persistent infection. Nature Microbiol., 5:1271-1284, 2020. PMID: 32661313
- News and Views Highlight: Prince, A., Staphylococcus aureus metabolites promote IL-10. Nature Microbiol., 5:1183–1184, 2020.