Catherine Gebhart, Ph.D.

Department Affiliations
Assistant Professor 
Director, Histocompatibility Laboratory
Technical Director, Molecular Diagnostics Laboratory

Interests
Histocompatibility testing for solid organ and bone marrow transplantation, infectious disease monitoring of immunocompromised individuals

I serve as Director of the Histocompatibility section of the Molecular Diagnostics Laboratory at Nebraska Medicine.  I am accredited by The American Board of Histocompatibility and Immunogenetics (ABHI) in Solid Organ and Bone Marrow Transplantation, and Histocompatibility Testing for Other Purposes.  The laboratory has recently completed the implementation of the first of two key process improvement projects.  mTilda, is a computerized lab information system, specifically designed to meet the unique needs of histocompatibility laboratories.  This has allowed for generation of concise summary reports available in an easily interpretable format to our transplant teams.  Furthermore, mTilda allows the laboratory to store and query original raw data, promoting synergy among clinical, education and research missions at Nebraska Medicine.  The second project, currently underway, is validation of NGS technology for high resolution typing of Human Leukocyte Antigen (HLA) genes.  This technology represents a major advancement in the field, enabling unambiguous allele assignment from a single run on a single platform, opposed to previous methods requiring maintenance of three separate platforms.  This will also streamline HLA typing of both bone marrow and solid organ patients.

I also serve as Technical Director of the Molecular Diagnostics Laboratory.  My primary responsibilities as Technical director include the daily supervision of the technical aspects of molecular virology testing in support of the transplant program for Nebraska Medicine. These responsibilities include quality control and assurance, problem solving and compliance for accreditation. I work in collaboration with the Medical Director and associated pathologists to evaluate and validate new testing.

I advocate for the development, improvement and streamlining of technology in order to provide patients with the best possible health care.  Outside of my laboratory responsibilities, I serve as an advisory board member for the Nebraska Organ Recovery System and as the Region 8 representative to the UNOS Histocompatibility committee.  I am actively involved in both ASCP molecular technologist education initiatives as well as ABHI technologist exam committees.

Education and Training

B.S., Creighton University, 1992
Ph.D., Pathology & Microbiology, UNMC, 1998
Postdoctoral Fellow, Pediatrics, Creighton University, 1998
Postdoctoral Fellow, Pharmaceutical Sciences, UNMC, 1998-2000
Technologist in Molecular Pathology, MP (ASCP), 2005
Diplomate,  American Board of Histocompatibility and Immunogenetics D(ABHI), 2010

Teaching Activities
Course co-coordinator, PAMM 509, Immunology for Pharmacy Students, UNMC
M-ID-720, Of Molecules and Mankind: The Role of the Clinical Laboratory in Patient Care, UNMC
PAMM 955 Advanced Immunobiology, UNMC

Faculty Advisor:
Bushra Bakhsh, M.S. student                                                 2014-2016
Mohammad Awaji, M.S. student                                           2013-2015

National Activities
Member, American Society for Histocompatibility & Immunogenetics (ASHI)
Member, Association for Molecular Pathology (AMP)
Member, American Society for Clinical Pathology (ASCP)
Member, American Society of Transplantation (AST)
Laboratory Inspector, College of American Pathologists (CAP)
Laboratory Inspector, American Society for Histocompatibility & Immunogenetics (ASHI)
Editorial Board, Lab Medicine
Committee Member, American Board of Histocompatibility and Immunogenetics (ABHI) CHS and CHA/CHT exam programs
2007-2013 ASCP Molecular Pathology Examination Committee of the Board of Registry; Committee Chair 2011-2013.

Notable Publications
Eugenia Raichlin E, Um J, Duncan K, Dumitru I, Lowes B, Moulton M, Gebhart CL, Grant W, Hammel J. Combined Heart and Liver Transplantation against Positive Cross-match for Patient with Hypoplastic Left Heart Syndrome.Transplantation 2014, 98(12).4).

Bathla L, Grant WJ, Mercer DF, Vargas LM, Gebhart CL, Langnas AN.  Parvovirus Associated Fulminant Hepatic Failure and Aplastic Anemia Treated Successfully With Liver and Bone Marrow Transplantation.  A Report of Two Cases.  Am J Transplant 2014, Sep 1. Doi: 10.1111/ajt.12857. [Epub ahead of print]

Gorantla S, Makarov E, Finke-Dwyer J, Castanedo A, Holguin A, Gebhart CL, Gendelman H, Poluektova LY.  Links between progressive HIV-1 infection of humanized mice and viral neuropathogenesis.  Am J Pathol 2010, 177(6), 2938-49.

Gorantla S, Makarov E, Finke-Dwyer J, Gebhart CL, Domm W, Dewhurst S, Gendelman H, Poluektova LY.  CD8+ cell depletion accelerates HIV-1 immunopathology in humanized mice.  J Immunol 2010, 184(12), 7082-91.

Levitsky J, Freifeld AG, Puumala S, Bargenquast K, Hardiman P, Gebhart C, Wrenshall L, Langnas A, Kalil AC.  Cytomegalovirus Viremia in Solid Organ Transplantation:  Does the Initial Viral Load Correlate with Risk Factors and Outcomes?   Clin Transplant  2008,  22, 222-228.

Gerriets JE, Greiner TC, Gebhart CL. Implementation of a T4 Extraction Control for Molecular Assays of Cerebral Spinal Fluid and Stool Specimens.  J Mol Diagn   2008, 10, 28-32.

Sriadibhatla, S, Yang Z, Gebhart, C., Alakhov, VY, Kabanov, A.  Transcriptional Activation of Gene Expression by Pluronic Block Copolymers in Stably and Transiently Transfected Cells.  Molecular Therapy, 2006, 13(4), 804-813. 

Yang Z, Zhu J, Sriadibhatla S, Gebhart CL, Zlakhov V, Kabanov A.  Promoter- and strain-selective enhancement of gene expression in a mouse skeletal muscle by a polymer excipient Pluronic P85.  Journal of Controlled Release, 2005, 108, 496-512.

Gebhart CL, Kabanov AV. Perspectives in Polymeric Gene Delivery. J Bioactive and Compatible Polymers 2003, 18,147-166.

Gebhart CL, Sriadibhatla S, Vinogradov S, Lemieux P, Alakhov V, Kabanov AV. Design and Formulation of Polyplexes Based on PluronicÒ-polyethyleneimine Conjugates for Gene Transfer. Bioconj Chem 2002, 13, 937-944.

Gebhart CL, Kabanov AV. Evaluation of Polyplexes as Gene Transfer Agents. J. Contr. Rel. 2001, 73, 401-416.

Nguyen HK, Lemieux P, Vinogradov S, Gebhart CL, Guérin N, Paradis G, Bronich T, Alakhov V Yu, Kabanov AV. Evaluation of Polyether-polyethyleneimine Graft Copolymers as Gene Transfer Agents. Gene Therapy 2000, 7, 126-138.

Lemieux P, Vinogradov SV, Gebhart CL, Guérin N, Paradis G, Nguyen H-K, Ochietti B, Suzdaltseva YG, Bartakova EV, Bronich TK, St-Pierre Y, Alakhov V Yu, Kabanov AV. Block and Graft Copolymers and NanogelÔ Copolymer Networks for DNA Delivery into Cell. J. Drug Targeting 2000, 8, 91-105.

Talmadge JE, Reed EC, Kessinger A, Kuszynski CA, Perry GA, Gordy CL, Mills KC, Thomas ML, Pirruccello SJ, Letheby BA, Arneson MA, Jackson JD. Immunologic Attributes of Cytokine Mobilized Peripheral Blood Stem Cells and Recovery Following Transplantation. Bone Marrow Transplant, 1996, 17, 101-109.

Miyagawa H, Okada C, Sugiyama H, Hopp RJ, Agrawal DK, Nabe M, Gordy CL, Bewtra AK, Townley RG. Density Distribution and Density Conversion of Neutrophils in Allergic Subjects. Int Arch Allergy Appl Immunol, 1990, 93, 8-13.

For a detailed list of publications, click here.