Mariano Sanchez-Lockhart, Ph.D.

Department Affiliations
Assistant Professor

Interests:
The overarching goal of my research is to characterize the host:pathogen interaction with special emphasis on the immune response to vaccination or pathogen infection. The Center for Genome Sciences (CGS) utilized genomics tool to perform genotyping (MHC and SNPs analysis); epitope mapping of the humoral response, and B and T cell repertoire analysis by VDJ rearrangement using high-throughput sequencing. These tolls are designed to complement and improve the information obtained from conventional immunological assays, and are collectively called: Immunomics. These agnostic tools are used in animal models (mice and non-human primates), in human samples (either from vaccination trials or survivors from outbreaks) and also in the reservoir of the pathogen of interest (Egyptian rousette bats, the reservoir for Marburg and Rav viruses).

With the better understanding of the host response to the pathogen we aim to define immunological biomarkers for protection and disease prognosis, develop diagnosis tools, and improve vaccine design.

The Immunomic group, within the CGS, is a join collaboration between National Strategic Research Institute (NSRI), the University of Nebraska Medical Center (UNMC) and the U.S. Army Medical Research Institute of Infectious Diseases (USAMRIID), lead Department of Defense (DoD) laboratory for biodefense research. The NSRI, UNMC and USAMRIID have partner to study dangerous pathogens, understand the key features of the immune response, and use those knowledge to develop therapeutics and vaccines.

Education and Training
B.Sc., School of Pharmacy and Biochemistry. University of Buenos Aires. Buenos Aires. Argentina, 1992
Ph.D., Dept. Immunology (IDEHU). University of Buenos Aires. Buenos Aires. Argentina., 1999
Postdoctoral training, Dept. Molecular Genetics & Cell Biology. University of Chicago. Chicago. IL, 1999-2002
Postdoctoral training, Dept. Microbiology & Immunology. University of Rochester. Rochester. NY, 2002-2005
Research Scientist, Dept. Microbiology & Immunology. University of Rochester. Rochester. NY, 2005-2012
Director of Immunomics. Center for Genomic Sciences. U.S. Army Medical Research Institute of Infectious Diseases (USAMRIID), 2012/present
Assistant Professor, Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, NE, 2012/present

Teaching Activities
Advanced Immunology (PATH 955)

Notable Publications
Sanchez-Lockhart M, Reyes DS, Gonzalez JC, Garcia KY, Villa EC, Pfeffer BP, Trefry JC,   Kugelman JR, Pitt ML and Palacios G. Qualitative profiling of the humoral immune response elicited by rVSV-DG-EBOV-GP using a systems serology assay, Domain Programmable Arrays. Cell Reports 2018. On print.

Pavlovich SS, Lovett SP, Koroleva G, Guito JC, Arnold CE, Nagle ER, Kulcsar K, Lee A, Thibaud-Nissen F, Hume AJ, Muhlberger E, Uebelhoer LS, Towner JS, Rabadan R, Sanchez-Lockhart M, Kepler TB, Palacios G. The Egyptian Rousette Genome Reveals Unexpected Features of Bat Antiviral Immunity. Cell. 2018; 173(5):1098-110.

Dudas G, Carvalho LM, Bedford T, Tatem AJ, Baele G, Faria NR, et al. Virus genomes reveal factors that spread and sustained the Ebola epidemic. Nature. 2017; 544(7650):309-15.

Blackley DJ, Wiley MR, Ladner JT, Fallah M, Lo T, Gilbert ML, Gregory C, D'ambrozio J, Coulter S, Mate S, Balogun Z, Kugelman J, Nwachukwu W, Prieto K, Yeiah A, Amegashie F, Kearney B, Wisniewski M, Saindon J, Schroth G, Fakoli L, Diclaro JW 2nd, Kuhn JH, Hensley LE, Jahrling PB, Ströher U, Nichol ST, Massaquoi M, Kateh F, Clement P, Gasasira A, Bolay F, Monroe SS, Rambaut A, Sanchez-Lockhart M, Scott Laney A, Nyenswah T, Christie A, Palacios G. Reduced evolutionary rate in reemerged Ebola virus transmission chains. Sci Adv. 2016; 2(4):e1600378

Yu GY, Mate S, Garcia K, Ward MD, Brueggemann E, Hall M, Kenny T, Sanchez-Lockhart M, Lefranc MP, Palacios G. Cynomolgus macaque (Macaca fascicularis) immunoglobulin heavy chain locus description. Immunogenetics. 2016; 68(6-7):417-28.

Ladner JT, Wiley MR, Mate S, Dudas G, Prieto K, Lovett S, Nagle ER, Beitzel B, Gilbert ML, Fakoli L, Diclaro JW, 2nd, Schoepp RJ, Fair J, Kuhn JH, Hensley LE, Park DJ, Sabeti PC, Rambaut A, Sanchez-Lockhart M, Bolay FK, Kugelman JR, Palacios G. Evolution and Spread of Ebola Virus in Liberia, 2014-2015. Cell Host Microbe. 2015; 18(6):659-69.

Lee AK, Kulcsar KA, Elliott O, Khiabanian H, Nagle ER, Jones ME, Amman BR, Sanchez-Lockhart M, Towner JS, Palacios G, Rabadan R. De novo transcriptome reconstruction and annotation of the Egyptian rousette bat. BMC Genomics. 2015; 16:1033.

Mate SE, Kugelman JR, Nyenswah TG, Ladner JT, Wiley MR, Cordier-Lassalle T, Christie A, Schroth GP, Gross SM, Davies-Wayne GJ, Shinde SA, Murugan R, Sieh SB, Badio M, Fakoli L, Taweh F, de Wit E, van Doremalen N, Munster VJ, Pettitt J, Prieto K, Humrighouse BW, Stroher U, DiClaro JW, Hensley LE, Schoepp RJ, Safronetz D, Fair J, Kuhn JH, Blackley DJ, Laney AS, Williams DE, Lo T, Gasasira A, Nichol ST, Formenty P, Kateh FN, De Cock KM, Bolay F, Sanchez-Lockhart M, Palacios G. Molecular Evidence of Sexual Transmission of Ebola Virus. N Engl J Med. 2015; 373(25):2448-54

Kugelman JR, Kugelman-Tonos J, Ladner JT, Pettit J, Keeton CM, Nagle ER, Garcia KY, Froude JW, Kuehne AI, Kuhn JH, Bavari S, Zeitlin L, Dye JM, Olinger GG, Sanchez-Lockhart M, Palacios GF. Emergence of Ebola Virus Escape Variants in Infected Nonhuman Primates Treated with the MB-003 Antibody Cocktail. Cell Rep. 2015; 12(12):2111-20.

Westbrook CJ, Karl JA, Wiseman RW, Mate S, Koroleva G, Garcia K, Sanchez-Lockhart M, O'Connor DH, Palacios G. No assembly required: Full-length MHC class I allele discovery by PacBio circular consensus sequencing. Hum Immunol. 2015; 76(12):891-6.

 

 

For a detailed list of publications, click here