Mariano Sanchez-Lockhart, Ph.D.

Department Affiliations
Assistant Professor

The overarching goal of my research is to characterize the humoral and cellular immune response to pathogen infection or vaccination challenge. The Center for Genome Sciences utilized genomics tool to perform genotyping (MHC and SNPs analysis); epitope mapping of the humoral response (phage display systems platform) and B and T cell repertoire analysis by VDJ rearrangement using high-throughput sequencing.

Using these Immunomic tools we aim to define immunological biomarkers for protection and disease prognosis, develop diagnosis tools, and improve vaccine designs.

The Center for Genome Sciences is located at USAMRIID (U.S. Army Medical Research Institute of Infectious Diseases), a lead Department of Defense (DoD) laboratory for biodefense research. The UNMC and USAMRIID have partner to study dangerous pathogens, understand the key features of the immune response, and use those knowledge to develop therapeutics and vaccines.

Education and Training
B.Sc., School of Pharm. and Biochemistry. University of Buenos Aires. Buenos Aires. Argentina, 1992
Ph.D., Dept. Immunology (IDEHU). University of Buenos Aires. Buenos Aires. Argentina. 1994-1999
Postdoctoral training, Argentina National Research Council (CONICET). External fellowship - Argentina. Dept. Molecular Genetics & Cell Biology. University of Chicago. Chicago. IL, 1999-2002
Postdoctoral training, Dept. Microbiology & Immunology. University of Rochester. Rochester. NY 2002-2005
Research Scientist, Dept. Microbiology & Immunology. University of Rochester. Rochester. NY 2005-2012
Assistant Professor, Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, NE

Notable Publications

Ladner JT, Wiley MR, Mate S, Dudas G, Prieto K, Lovett S, Nagle ER, Beitzel B, Gilbert ML, Fakoli L, Diclaro JW, 2nd, Schoepp RJ, Fair J, Kuhn JH, Hensley LE, Park DJ, Sabeti PC, Rambaut A, Sanchez-Lockhart M, Bolay FK, Kugelman JR, Palacios G. 2015. Evolution and Spread of Ebola Virus in Liberia, 2014-2015. Cell Host Microbe 18:659-669.

Lee AK, Kulcsar KA, Elliott O, Khiabanian H, Nagle ER, Jones ME, Amman BR, Sanchez-Lockhart M, Towner JS, Palacios G, Rabadan R. 2015. De novo transcriptome reconstruction and annotation of the Egyptian rousette bat. BMC Genomics 16:1033.

Mate SE, Kugelman JR, Nyenswah TG, Ladner JT, Wiley MR, Cordier-Lassalle T, Christie A, Schroth GP, Gross SM, Davies-Wayne GJ, Shinde SA, Murugan R, Sieh SB, Badio M, Fakoli L, Taweh F, de Wit E, van Doremalen N, Munster VJ, Pettitt J, Prieto K, Humrighouse BW, Stroher U, DiClaro JW, Hensley LE, Schoepp RJ, Safronetz D, Fair J, Kuhn JH, Blackley DJ, Laney AS, Williams DE, Lo T, Gasasira A, Nichol ST, Formenty P, Kateh FN, De Cock KM, Bolay F, Sanchez-Lockhart M, Palacios G. 2015. Molecular Evidence of Sexual Transmission of Ebola Virus. N Engl J Med 373:2448-2454.

Kugelman JR, Kugelman-Tonos J, Ladner JT, Pettit J, Keeton CM, Nagle ER, Garcia KY, Froude JW, Kuehne AI, Kuhn JH, Bavari S, Zeitlin L, Dye JM, Olinger GG, Sanchez-Lockhart M, Palacios GF. 2015. Emergence of Ebola Virus Escape Variants in Infected Nonhuman Primates Treated with the MB-003 Antibody Cocktail. Cell Rep 12:2111-2120.

Kugelman JR, Wiley MR, Mate S, Ladner JT, Beitzel B, Fakoli L, Taweh F, Prieto K, Diclaro JW, Minogue T, Schoepp RJ, Schaecher KE, Pettitt J, Bateman S, Fair J, Kuhn JH, Hensley L, Park DJ, Sabeti PC, Sanchez-Lockhart M, Bolay FK, Palacios G, Diseases USAMRIoI, National Institutes of H, Integrated Research Facility-Frederick Ebola Response T. 2015. Monitoring of Ebola Virus Makona Evolution through Establishment of Advanced Genomic Capability in Liberia. Emerg Infect Dis 21:1135-1143.

Westbrook CJ, Karl JA, Wiseman RW, Mate S, Koroleva G, Garcia K, Sanchez-Lockhart M, O'Connor DH, Palacios G. 2015. No assembly required: Full-length MHC class I allele discovery by PacBio circular consensus sequencing. Hum Immunol doi:10.1016/j.humimm.2015.03.022.

Kugelman JR, Sanchez-Lockhart M, Andersen KG, Gire S, Park DJ, Sealfon R, Lin AE, Wohl S, Sabeti PC, Kuhn JH, Palacios GF. 2015. Evaluation of the potential impact of ebola virus genomic drift on the efficacy of sequence-based candidate therapeutics. MBio 6.

Sanchez-Lockhart M, Rojas AV, Fettis MM, Bauserman R, Higa TR, Miao H, Waugh RE, Miller J. 2014. T cell receptor signaling can directly enhance the avidity of CD28 ligand binding. PLoS One 9:e89263.

For a detailed list of publications, click here