Muthukumar Kannan, PhD
Laboratory: Shilpa Buch, PhD
University of Nebraska Medical Center
985880 Nebraska Medical Center
Omaha Nebraska, NE 68198-5880
Keywords: HIV-1, Iron metabolism, Drugs of abuse, Lipids, Organelle biogenesis.
In my post-doctoral training, I investigated the regulation of sphingolipid synthesis and the mechanism of intracellular lipid transport. I recently identified a novel mechanism by which cells promote lipid transfer at membrane contact sites, and the findings were published in reputed journals like Developmental cell, Journal of Lipid Research, and Biochimica et Biophysica Acta (BBA). After gaining research expertise in lipid metabolism, I sought to enter the area of neuroscience in the context of HIV and drug abuse. After joining Dr. Buch’s team, I am now applying my lipid expertise to neuroHIV and drug abuse. I am studying the exacerbation of neuroinflammation by iron-mediated lipid peroxidation (Ferroptosis) in a rodent model of HIV and drug abuse.
- Kannan M, Singh S, Chemparathy DT, Oladapo AA, Gawande DY, Dravid SM, Buch S, Sil S (2022) HIV-1 Tat induced microglial EVs leads to neuronal synaptodendritic injury: microglia-neuron cross-talk in NeuroHIV. Extracell Vesicles Circ Nucleic Acids 2022;3:133-49.
- Chemparthy DT, Kannan M, Gordon L, Buch S, Sil S (2021) Alzheimer's-Like Pathology at the Crossroads of HIV-Associated Neurological Disorders. Vaccines. 2021 Aug 21;9(8):930.
- Sil S, Thangaraj A, Chivero ET, Niu F, Kannan M, Liao K, Silverstein PS, Periyasamy P, Buch S. HIV-1 and drug abuse comorbidity: Lessons learned from the animal models of NeuroHIV. Neurosci Lett. 2021 May 29;754:135863
- Kannan M, Davis DL, Suemitsu J, Oltorik, CD and Wattenberg B. (2020) Preparation of HeLa Total Membranes and Assay of Lipid-inhibition of Serine Palmitoyltransferase Activity. Bio-protocol 10(12): e3656.
- Davis D, Kannan M and Wattenberg B. (2018) Orm/ORMDL proteins: Gate guardians and master regulators. Adv Biol Regul. 70:3-18.
- Kannan M, Lahiri S, Liu LK, Choudhary V, Prinz WA. (2017) Phosphatidylserine synthesis at membrane contact sites promotes its transport out of the ER. J. Lipid Res. 58:553-562.
- Kannan M, Sivaprakasam C, Prinz WA, Nachiappan V. (2016) Endoplasmic reticulum stress disturbs the transport of phosphatidylethanolamine from mitochondria to endoplasmic reticulum in yeast cells. Biochim Biophys Acta. 1861:1959-1967.
- Friedman F.R, Kannan M, Toulmay A, Calvin H. Jan C.H, Weissman S, Prinz W.A, Nunnari J. (2018) Lipid homeostasis is maintained by dual targeting of the mitochondrial PE biosynthesis enzyme to the ER. Developmental Cell. 44:261-270.e6. DOI: 10.1016/j.devcel.2017.11.023.
- Kannan M, Riekhof WR, Voelker DR. (2015) Transport of Phosphatidylserine from the Endoplasmic Reticulum to the Site of Phosphatidylserine Decarboxylase2 in Yeast. 16(2):123-34.
- Muthukumar K, Nachiappan V. (2013) Phosphatidylethanolamine from phosphatidylserine decarboxylase2 is essential for autophagy under cadmium stress in Saccharomyces cerevisiae. Cell Biochem Biophys. 67(3):1353-63.