Director, Mouse Genome Engineering Core Facility
Durham Research Center II 1030/8187
985930 Nebraska Medical Center
Omaha, NE 68198-5930
Keywords: genome editing technology development and animal model designing
Notable contributions: While serving as the core director, and in collaboration with Dr. Masato Ohtsuka, Tokai University, Japan, Guru’s research work has developed many breakthrough technologies. These include Easi-CRISPR[I, II], GONAD[III, IV], improved-GONAD[V, VI], CRISPR-First, PITT-next[VII], Pronuclear Injection based Targeted Transgenesis PITT[VIII, IX], and improved-PITT[X]. The research community regards Easi-CRISPR and GONAD contributions as “breakthroughs that have redefined the transgenic technologies practiced for the last four decades”[XI].
A short video about Easi-CRISPR and GONAD technologies is here.
UNMC Today | February 17, 2021
UNMC team contributes to study on 'sixth sense'
UNMC Today | February 8, 2021
Distinguished Scientist: Channabasavaiah Gurumurthy, PhD
UNMC Today | December 3, 2020
'Designer' mice blueprints to aid COVID-19 research
Omaha Magazine | January 2, 2020
Using Cut-And-Paste To Edit Out Human Disease
UNMC Today | August 28, 2019
Global team evaluates CRISPR gene editing methods
NIH News Release | August 27, 2019
NHGRI announces six inaugural Genomic Innovator Awards
UNMC Today | August 7, 2019
GONAD recipe published in Nature Protocols
UNMC Today | July 17, 2019
Research team makes ophthalmology breakthrough
UNMC Today | April 29, 2019
Dr. Gurumurthy elected to international transgenic board
CB Gurumurthy received his BVSC (DVM) from Bangalore Veterinary College, India and MVSC & PHD in Veterinary Virology from Indian Veterinary Research Institute with a Gold Medal distinction in MVSC. He received post-doctoral training in Cancer Biology and Mouse Molecular Genetics at Northwestern University, Chicago. He obtained a faculty position at the University of Nebraska Medical Center, Omaha in 2007 and since 2010 he serves as the Director of UNMC Mouse Genome Engineering Core Facility. He also has an Executive MBA degree, obtained from University of Nebraska at Omaha. His passion is genome engineering and technology development. With his experience both in traditional and advanced genetic engineering techniques, he is actively developing newer technologies for generation of genetically engineered animal models and developing widely-usable animal models for basic and drug discovery research.
Genome engineering technologies that remained relatively unchanged for nearly 3 decades have taken on a new life, especially by the addition of CRISPR/Cas9 technology to the toolbox. With my training, experience and knowledge gained in designing and developing genetically engineered animal models, my laboratory focuses on the following two research areas:
- Development and improvement of transgenic and genome editing technologies.
During the recent couple years, in collaboration with Dr. Masato Ohtsuka, Tokai University Japan, our laboratory has developed several genome editing and transgenic technology tools such as PITT (Pronuclear Injection based Targeted Transgenesis), CRISPR-First;PITT-next, GONAD (Genome Editing via Oviductal Nucleaic Acids Delivery) and Easi-CRISPR. We are actively working on one of the biggest challenges of genome editing technologies; enhancing the insertion efficiency of repair DNA molecules using the CRISPR system.
- Development of versatile animal model resources and tools for a wide range of research areas.
We design and develop animal models for various biomedical research projects. Some examples of on-going projects that can generate widely usable animal model resources are 1) Reporter-Linked-Cre (RePliCre) mouse models using PITT-Plus technology and 2) Development of humanized animal models.
- Miura H, Imafuku J, Kurosaki A, Sato M, Ma Y, Zhang G, Mizutani A, Kamimura K, Gurumurthy CB, Liu D, Ohtsuka M. Novel reporter mouse models useful for evaluating in vivo gene editing and for optimization of methods of delivering genome editing tools. Mol Ther Nucleic Acids. 2021 Mar 5;24:325-336. doi: 10.1016/j.omtn.2021.03.003. PMID: 33850636. PMCID: PMC8020343.
- Gurumurthy CB, Saunders TL, Ohtsuka M. Designing and generating a mouse model: frequently asked questions. J Biomed Res. 2021 Mar 26;35(2):76-90. doi: 10.7555/JBR.35.20200197. PMID: 33797414. PMCID: PMC8038528.
- Gurumurthy CB*, Quadros RM, Richardson GP, Poluektova LY, Mansour SL, Ohtsuka M*. Genetically modified mouse models to help fight COVID-19. Nat Protoc. 2020 Oct 26. doi: 10.1038/s41596-020-00403-2. PMID: 33106680. PMCID: PMC7704938.
- Gurumurthy CB*, O'Brien AR, Quadros RM, Adams J Jr, Alcaide P, Ayabe S, Ballard J, Batra SK, Beauchamp MC, Becker KA, Bernas G, Brough D, Carrillo-Salinas F, Chan W, Chen H, Dawson R, DeMambro V, D'Hont J, Dibb KM, Eudy JD, Gan L, Gao J, Gonzales A, Guntur AR, Guo H, Harms DW, Harrington A, Hentges KE, Humphreys N, Imai S, Ishii H, Iwama M, Jonasch E, Karolak M, Keavney B, Khin NC, Konno M, Kotani Y, Kunihiro Y, Lakshmanan I, Larochelle C, Lawrence CB, Li L, Lindner V, Liu XD, Lopez-Castejon G, Loudon A, Lowe J, Jerome-Majewska LA, Matsusaka T, Miura H, Miyasaka Y, Morpurgo B, Motyl K, Nabeshima YI, Nakade K, Nakashiba T, Nakashima K, Obata Y, Ogiwara S, Ouellet M, Oxburgh L, Piltz S, Pinz I, Ponnusamy MP, Ray D, Redder RJ, Rosen CJ, Ross N, Ruhe MT, Ryzhova L, Salvador AM, Alam SS, Sedlacek R, Sharma K, Smith C, Staes K, Starrs L, Sugiyama F, Takahashi S, Tanaka T, Trafford AW, Uno Y, Vanhoutte L, Vanrockeghem F, Willis BJ, Wright CS, Yamauchi Y, Yi X, Yoshimi K, Zhang X, Zhang Y, Ohtsuka M, Das S, Garry DJ, Hochepied T, Thomas P, Parker-Thornburg J, Adamson AD, Yoshiki A, Schmouth JF, Golovko A, Thompson WR, Lloyd KCK, Wood JA, Cowan M, Mashimo T, Mizuno S, Zhu H, Kasparek P, Liaw L, Miano JM, Burgio G*. Genome Biol. 2019 Aug 26;20(1):171. doi: 10.1186/s13059-019-1776-2. PMCID: PMC6709553
- Gurumurthy CB*, Masahiro Sato, Ayaka Nakamura, Masafumi Inui, Natsuko Kawano, Md Atiqul Islam, Sanae Ogiwara, Shuji Takabayashi, Makoto Matsuyama, Shinichi Nakagawa, Hiromi Miura, Masato Ohtsuka*; Creating CRISPR-based germline genome engineered mice without ex vivo handling of zygotes by i-GONAD, Nature Protoc. 2019, doi. 10. 1038/s41596-019-0187-x, PMID: 31341289
- Gurumurthy CB, Lloyd KCK. Generating mouse models for biomedical research: technological advances. Dis Model Mech. 2019 Jan 8;12(1). doi: 10.1242/dmm.029462. Review. PubMed PMID: 30626588
- Miura H, Quadros RM, Gurumurthy CB*, Ohtsuka M*. Easi-CRISPR for creating knock-in and conditional knockout mouse models using long ssDNA donors. Nat Protoc. 2018 Jan;13(1):195-215. doi: 10.1038/nprot.2017.153. PMID: 29266098
- Quadros RM, Miura H, Harms DW, Akatsuka H, Sato T, Aida T,Redder R, Richardson GP, Inagaki Y, Sakai D, Buckley SM, Seshacharyulu P, Batra SK, Behlke MA, Zeiner SA, Jacobi AM, Izu Y, Thoreson WB, Urness LD, Mansour SL, Ohtsuka M, Gurumurthy CB. Easi-CRISPR: a robust method for one-step generation of mice carrying conditional and insertion alleles using long ssDNA donors and CRISPR ribonucleoproteins. Genome Biol. 2017;18:92. PMID: 28511701
- Ohtsuka M, Sato M, Miura H, Takabayashi S, Matsuyama M, Koyano T, Arifin N, Nakamura S, Wada K, Gurumurthy CB. i-GONAD: a robust method for in situ germline genome engineering using CRISPR nucleases. Genome Biol. 2018;19:25. PMID: 29482575
- Quadros RM, Harms DW, Ohtsuka M, Gurumurthy CB. Insertion of sequences at the original provirus integration site of mouse ROSA26 locus using the CRISPR/Cas9 system. FEBS Open Bio. 2015;5:191–7. PMID: 25834785
- Burgio G. Redefining mouse transgenesis with CRISPR/Cas9 genome editing technology. Genome Biol. 2018;19:27. PMID: 29490686
Full list of publications on PubMedreturn to top of page