Director, Mouse Genome Engineering Core Facility
Durham Research Center II 1030/8187
985930 Nebraska Medical Center
Omaha, NE 68198-5930
Keywords: genome editing technology development and animal model designing
Notable contributions: While serving as the core director, and in collaboration with Dr. Masato Ohtsuka, Tokai University, Japan, Guru’s research work has developed many breakthrough technologies. These include Easi-CRISPR[1, 2], GONAD[3, 4], improved-GONAD[5, 6], CRISPR-First, PITT-next, Pronuclear Injection based Targeted Transgenesis PITT[8, 9], and improved-PITT[2, 10]. The research community regards Easi-CRISPR and GONAD contributions as “breakthroughs that have redefined the transgenic technologies practiced for the last four decades” .
A short video about Easi-CRISPR and GONAD technologies is here.
UNMC Today | August 28, 2018
Global team evaluates CRISPR gene editing methods
NIH News Release | August 27, 2019
NIH announces six inaugural Genomic Innovator Awards
UNMC Today | August 7, 2019
GONAD recipe published in Nature Protocols
UNMC Today | July 17, 2019
Research team makes ophthalmology breakthrough
UNMC Today | April 29, 2019
Dr. Gurumurthy elected to international transgenic board
CB Gurumurthy received his BVSC (DVM) from Bangalore Veterinary College, India and MVSC & PHD in Veterinary Virology from Indian Veterinary Research Institute with a Gold Medal distinction in MVSC. He received post-doctoral training in Cancer Biology and Mouse Molecular Genetics at Northwestern University, Chicago. He obtained a faculty position at the University of Nebraska Medical Center, Omaha in 2007 and since 2010 he serves as the Director of UNMC Mouse Genome Engineering Core Facility. He also has an Executive MBA degree, obtained from University of Nebraska at Omaha. His passion is genome engineering and technology development. With his experience both in traditional and advanced genetic engineering techniques, he is actively developing newer technologies for generation of genetically engineered animal models and developing widely-usable animal models for basic and drug discovery research.
Genome engineering technologies that remained relatively unchanged for nearly 3 decades have taken on a new life, especially by the addition of CRISPR/Cas9 technology to the toolbox. With my training, experience and knowledge gained in designing and developing genetically engineered animal models, my laboratory focuses on the following three research areas:
- Development and improvement of transgenic and genome editing technologies.
During the recent couple years, in collaboration with Dr. Masato Ohtsuka, Tokai University Japan, our laboratory has developed several genome editing and transgenic technology tools such as PITT (Pronuclear Injection based Targeted Transgenesis), CRISPR-First;PITT-next, GONAD (Genome Editing via Oviductal Nucleaic Acids Delivery) and Easi-CRISPR. We are actively working on one of the biggest challenges of genome editing technologies; enhancing the insertion efficiency of repair DNA molecules using the CRISPR system.
- Development of versatile animal model resources and tools for a wide range of research areas.
We design and develop animal models for various biomedical research projects. Some examples of on-going projects that can generate widely usable animal model resources are 1) Reporter-Linked-Cre (RePliCre) mouse models using PITT-Plus technology and 2) Development of humanized animal models.
- Quadros RM, Miura H, Harms DW, Akatsuka H, Sato T, Aida T,Redder R, Richardson GP, Inagaki Y, Sakai D, Buckley SM, Seshacharyulu P, Batra SK, Behlke MA, Zeiner SA, Jacobi AM, Izu Y, Thoreson WB, Urness LD, Mansour SL, Ohtsuka M, Gurumurthy CB. Easi-CRISPR: a robust method for one-step generation of mice carrying conditional and insertion alleles using long ssDNA donors and CRISPR ribonucleoproteins. Genome Biol. 2017;18:92. PMID: 28511701
- Miura H, Quadros RM, Gurumurthy CB, Ohtsuka M. Easi-CRISPR for creating knock-in and conditional knockout mouse models using long ssDNA donors. Nat Protoc. 2017;13:195. PMID: 29266098
- Takahashi G, Gurumurthy CB, Wada K, Miura H, Sato M, Ohtsuka M. GONAD: Genome-editing via Oviductal Nucleic Acids Delivery system: a novel microinjection independent genome engineering method in mice. Sci Rep. 2015;5:11406. PMID: 26096991
- Gurumurthy CB, Takahashi G, Wada K, Miura H, Sato M, Ohtsuka M. GONAD: A Novel CRISPR/Cas9 Genome Editing Method that Does Not Require Ex Vivo Handling of Embryos: GONAD: A Novel CRISPR/Cas9 Genome Editing Method. In: Haines JL, Korf BR, Morton CC, Seidman CE, Seidman JG, Smith DR, editors. Curr Protoc Hum Genet [Internet]. Hoboken, NJ, USA: John Wiley & Sons, Inc.; 2016 [cited 2016 May 16]. p. 15.8.1-15.8.12. PMID: 26724720
- Gurumurthy CB, Sato M, Nakamura A, Inui M, Kawano N, Islam MA, et al. Creation of CRISPR-based germline-genome-engineered mice without ex vivo handling of zygotes by i-GONAD. Nat Protoc [Internet]. 2019. PMID: 31341289
- Ohtsuka M, Sato M, Miura H, Takabayashi S, Matsuyama M, Koyano T, Arifin N, Nakamura S, Wada K, Gurumurthy CB. i-GONAD: a robust method for in situ germline genome engineering using CRISPR nucleases. Genome Biol. 2018;19:25. PMID: 29482575
- Quadros RM, Harms DW, Ohtsuka M, Gurumurthy CB. Insertion of sequences at the original provirus integration site of mouse ROSA26 locus using the CRISPR/Cas9 system. FEBS Open Bio. 2015;5:191–7. PMID: 25834785
- Ohtsuka M, Miura H, Sato M, Kimura M, Inoko H, Gurumurthy CB. PITT: pronuclear injection-based targeted transgenesis, a reliable transgene expression method in mice. Exp Anim Jpn Assoc Lab Anim Sci. 2012;61:489–502. PMID: 23095812
- Ohtsuka M, Miura H, Hayashi H, Nakaoka H, Kimura M, Sato M, Gurumurthy CB, Inoko H. Improvement of pronuclear injection-based targeted transgenesis (PITT) by iCre mRNA-mediated site-specific recombination. Transgenic Res. 2013;22:873–5. PMID: 23529203
- Ohtsuka M, Miura H, Mochida K, Hirose M, Hasegawa A, Ogura A, Mizutani R, Kimura M, Isotani A, Ikawa M, Sato M, Gurumurthy CB. One-step generation of multiple transgenic mouse lines using an improved Pronuclear Injection-based Targeted Transgenesis (i-PITT). BMC Genomics. 2015;16:274. PMID: 25887549
A commentary about Easi-CRISPR and GONAD technologies
- Burgio G. Redefining mouse transgenesis with CRISPR/Cas9 genome editing technology. Genome Biol. 2018;19:27. PMID: 29490686
Full list of publications on PubMed
Dr. Gurumurthy's Biographical Information
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