Work in my laboratory focus on the effects of HIV and secreted viral products on the vascular endothelium, how this and viral genotype influences HIV-mediated pathology in the brain and lung. Using a multipronged approach including in vitro culture system with primary human cells, archival human tissues, and in vivo HIV/AIDS mice models, research in my lab aimed to: 1) decipher the mechanisms mediating blood-brain barrier (BBB) dysfunction and virus invasion of the central nervous system (CNS) in HIV-associated neurocognitive disorders (HAND); 2) investigate the influence of viral genotype on HIV CNS infection and international neuroAIDS; 3) investigate how nanomedicine can help improve CNS penetrance of antiretroviral drugs and help eliminate viral reservoirs in the brain; and 4) investigate the mechanisms mediating HIV-induced injury of the pulmonary endothelium and its relation to pulmonary complications in infected individuals.
The long-range goals are to improve CNS delivery of antiviral drugs, determine the role of blood- or brain- derived HIV-1, viral genotype, and secreted viral products in the breach of BBB integrity and virus brain invasion; and to design therapeutics approaches to prevent HIV-1-mediated dysfunction of the endothelium and viral entry into the CNS and other organs such as the lungs.
Alfred K. Njamnshi, MD, MA, DMS, Chief Neurology Department. Yaoundé Central Hospital, University of Yaoundé I, Cameroon
Dora Mbanya, MD, PhD, Chief of Hematology and Transfusion Service, Yaoundé Teaching Hospital, Yaoundé Central Hospital, University of Yaoundé I, Cameroon
Robert K. Heaton, PhD, ABPP-CN, Professor of Psychiatry, Director, UCSD/SDSU Joint Doctoral Program in Clinical Psychology, Director, Neurobehavioral Core, HIV Neurobehavioral Research Center, UCSD School of Medicine
Mariana Cherner, PhD, Associate Professor of Psychiatry, Director Interdisciplinary Research Fellowship in NeuroAIDS, HIV Neurobehavioral Research Program, University of California, San Diego, CA
Howard Gendelman, MD, Department of Pharmacology and Experimental Neuroscience, University of Nebraska Medical Center
JoEllyn McMillian, PhD, Department of Pharmacology and Experimental Neuroscience, University of Nebraska Medical Center
Fang Yu, PhD, Associate Professor, College of Public Health, University of Nebraska Medical Center
HIV Genetic Diversity and Viral Neuropathogenesis
Role: Principle Investigator
Source: NIH/NIMH 1R01 MH094160-01A1
Goal: The major goal of this project is to study the linkage between viral genotype and the risk of developing HIV-associated neurocognitive disorders in HIV-infected individuals in Cameroon.
NanoArt Manufacture, Delivery, and Pharmacokinetics for Optimizing Drug Adherence
Role: PI: H. Gendelman; Core B: G.D. Kanmogne
Source: NIH/NIDA. 1P01DA028555-02
Goal: This is an integrative cross approach translational & multi-investigator program grant seeking to develop nanoformulated antiretroviral drug therapy from the bench to the patient.
- Cell and Tissue Culture
- ChIP and Luciferases Assays
- Cell Adhesion and Migration Assays
- Mice Reconstitution with Human Immune cells
- FACS analysis
- Laser Confocal Microscopy
- Gene Array Analyses
- ELISA and Reserve Transcriptase Assays
- Protein and Nucleic Acid Purification
- PCR, Reverse Transcription-PCR, and Real-Time PCR
- Southern, Northern, and Western blot analysis
Biju Bhargavan, PhD