Confocal Training Information-User Tips

Tips


Guidelines for Sample Preparation 

  • Glass slides with 170µm-thickness coverglass

  • Cover glass-bottom tissue culture dish for live imaging (MatTek or Willco)

  • Appropriate fluorescent dyes for confocal or fluorescent imaging

  • Appropriate mounting media for fixed samples and dye-free tissue culture media for live imaging

MatTek dishes

Limitations

Standard Operational Procedures

Confocal SOP - pdf file


User Fees* (effective September 1, 2009)

  UNMC**
Consultation/basic training Free
Confocal use (untrained user) $30/hr
Confocal use (trained user) $15/hr
Time lapse studies $10/hr
Time lapse studies (brightfield only) $5/hr
Processing/image analysis $10/hr
Unassisted image analysis Free
   
Supply costs variable

* All fees will be prorated to the nearest half hour
** member of the Department of Pharmacology and Experimental Neuroscience

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