1. All users must make an appointment by phone with the facility manager.
  2. The manager will stagger start times for users on the different microscopes, and ensure users don’t leave at the same time.
  3. Use on each instrument will be staggered so that there will be sufficient time between users for proper disinfection and cleaning.
  4. Syran wrap with a clean rubber band will be placed on each eyepiece by core personnel, to be removed and replaced after each user leaves
  5. In addition, after each use, the microscope eyepieces and all touch surfaces will be disinfected with 70% ethanol and Kim wipes.
  6. Users should not come to the UAMCF or be on campus if ill.
  7. All users entering the facility must don masks or face coverings over mouth and nose.
  8. All users entering will be immediately required to wash hands with warm water and soap before proceeding to the microscope room. Taped spaces on the floor will indicate where to stand for washing up.
  9. After washing hands, all users with then don gloves and safety goggles for the remainder of their time in the facility. When use of the microscope is completed, the users will deposit the reusable safety goggles in ready buckets with disinfecting solution.
  10. Only a single user per lab will be permitted per microscope.
  11. Only experienced users will be able to operate the equipment at present; no new users will be trained by core personnel. This will be subject to change as we evaluate the situation.
  12. A user who is done will call the facility manager verbally, and she will give authorization for the user to leave the room, remove goggles and deposit them in the disinfection bucket, remove gloves and wash hands, and leave the facility. Authorization is to ensure only one user leaves the facility at a time.
  13. The manager will disinfect door handles and facility surfaces after each user leaves
  14. Use “after hours” (evenings/weekends) will not be permitted at this time, because core personnel need to be present to ensure proper disinfection protocols are carried out.
  15. In the event that a user requires assistance, he/she will call core personnel, explain the issue and leave the microscope room and stand by the demarcated/taped lines on the floor while the personnel troubleshoot the problem
  16. Hours of operation will typically be 8 am to 4 pm (by phone appointment only, no drop-ins) with opportunities to book time in advance from 4 pm to 8 pm Tuesdays and Thursdays.
  17. Over the coming months, hours of operation and rules for use of the facility will be subject to modification based on assessments of the situation.
  18. For any special issues or concerns, feel free to contact us directly.


Acknowledgement of the Core Facility

All users of the Advanced Microscopy Core Facility are obligated to acknowledge the facility in formal publications and presentations which include data generated in the facility.  We also appreciate receiving reprints of such publications.  

Confocal Acknowledgment:  

The University of Nebraska Medical Center Advanced Microscopy Core Facility receives partial support from the National Institute for General Medical Science (NIGMS) INBRE - P20 GM103427 and COBRE - P30 GM106397 grants, as well as support from the National Cancer Institute (NCI) for The Fred & Pamela Buffett Cancer Center Support Grant- P30 CA036727, and the Nebraska Research Initiative.  This publication’s contents and interpretations are the sole responsibility of the authors.

Structured Illumination Microscopy (SIM)

1)   Open for use Monday Sept. 15. For $30/hour, users will be able to bring SIM samples to core facility personnel for acquisition of images and basic processing.

2)    Only core personnel will acquire images and process them (including dual/multi-channel alignments), but users will be able to view and choose the regions of interest that they wish to acquire on cover-slips.

3)    The core facility will provide basic processing and channel alignment, and will allow users to choose how they would like the data rendered and saved. Among these options are TIF images of a composite 3D SIM, individual TIFs of various z-sections, altered angles, planes, etc. as TIF images, 3D movies, etc. Please note: these image processing manipulations are complex and take time, yet they are an essential part of the SIM analysis thus are part of the core service (and charge). First-time users may contact the core director for initial consultation/advise prior to using the facility. For each laboratory, this consultation will be free the first time, but will be subject to time and availability of the director.

4)    Users may also request a consultation with the core director to analyze/interpret results obtained at the processing workstation. The fee will be $50/half an hour, and is dependent on the availability of the director, with no obligation for the director/facility to provide this extra service. 

Extra service: Users who wish further analysis/interpretation of their results at the core processing workstation may request a consultation with the core director (core facility fee will be $50/half an hour, and is dependent on the availability of the director, with no obligation for the director/facility to provide this extra service).

 In select circumstances, where additional expertise is required and goes beyond an additional consultation, if requested the director may agree to serve as a collaborator for a given project. This is not a regular service provided by the facility and is subject to the discretion, time and availability of the director.