Imaging Resources

SPECTRAL DATABASES

LEARNING MODULES:  GENERAL MICROSCOPY & IMAGE ANALYSIS 

OPEN-SOURCE IMAGE ANALYSIS/VIEWING 

FLUORESCENT PROTEINS, DYES, BUFFERS FOR SUPER RESOLUTION IMAGING

Chromotek Nanobodies(novel primary and secondary antibodies for higher resolution & cleaner images) for super-resolution imaging 

STORMreagents (a broad list of proteins, dyes, buffers, and associated references for PALM/STORM imaging from Nikon) 

ThermoFisher STORM products (list available antibody conjugates, nuclei acid stains, organelle probes, buffers and reagents for STORM imaging) 

ThermoFisher SIM products (list of available antibody conjugates, organelle, and DNA stains) 

SELECTED MANUSCRIPTS (SAMPLE PREPARATION)  

Optimized protocol for combined PALM-dSTORM imaging. Glushonkov O, Réal E, Boutant E, Mély Y, Didier P. Sci Rep. 2018 Jun 8;8(1):8749.  

Evaluation of fluorophores for optimal performance in localization-based super-resolution imaging. Dempsey GT, Vaughan JC, Chen KH, Bates M, Zhuang X.Nat Methods. 2011 

Twinkle, twinkle little star: photoswitchable fluorophores for super-resolution imaging. Chozinski TJ, Gagnon LA, Vaughan JC.FEBS Lett. 2014 Oct 1;588(19):3603-12. 

Photostable and photoswitching fluorescent dyes for super-resolution imaging. Minoshima M, Kikuchi K.J Biol Inorg Chem. 2017 Jul;22(5):639-652 

Super-resolution Microscopy for Biological Imaging. Yang Z, Samanta S, Yan W, Yu B, Qu J.Adv Exp Med Biol. 2021;3233:23-43. doi: 10.1007/978-981-15-7627-0_2. 

SELECTED MANUSCRIPTS (REVIEW ARTICLES: SIM, PALM, STORM)

Unraveling the Nanoscopic Organization and Function of Central Mammalian Presynapses With Super-Resolution Microscopy. Carvalhais LG, Martinho VC, Ferreiro E, Pinheiro PS.Front Neurosci. 2021 Jan 8;14:578409. doi: 10.3389/fnins.2020.578409. eCollection 2020.

Super-resolution microscopy approaches to nuclear nanostructure imaging. Cremer C, Szczurek A, Schock F, Gourram A, Birk U.Methods. 2017 Jul 1;123:11-32. doi: 10.1016/j.ymeth.2017.03.019. Epub 2017 Apr 6. 

Progress in quantitative single-molecule localization microscopy. Deschout H, Shivanandan A, Annibale P, Scarselli M, Radenovic A.Histochem Cell Biol. 2014 Jul;142(1):5-17. doi: 10.1007/s00418-014-1217-y. Epub 2014 Apr 20. 

Recent advances in super-resolution fluorescence imaging and its applications in biology. Han R, Li Z, Fan Y, Jiang Y.J Genet Genomics. 2013 Dec 20;40(12):583-95. doi: 10.1016/j.jgg.2013.11.003. Epub 2013 Nov 23. 

Imaging cells at the nanoscale. Cox S, Jones GE.Int J Biochem Cell Biol. 2013 Aug;45(8):1669-78. doi: 10.1016/j.biocel.2013.05.010. Epub 2013 May 18. 

Digging Deeper: Advancements in Visualization of Inhibitory Synapses in Neurodegenerative Disorders. Radulović S, Sunkara S, Maurer C, Leitinger G.Int J Mol Sci. 2021 Nov 18;22(22):12470. doi: 10.3390/ijms222212470. 

Microclusters as T Cell Signaling Hubs: Structure, Kinetics, and Regulation. Balagopalan L, Raychaudhuri K, Samelson LE.Front Cell Dev Biol. 2021 Jan 26;8:608530. doi: 10.3389/fcell.2020.608530. eCollection 2020. 

Optical Microscopy and the Extracellular Matrix Structure: A Review. Poole JJA, Mostaço-Guidolin LB.Cells. 2021 Jul 12;10(7):1760. doi: 10.3390/cells10071760. 

Suborganellar Localization of Mitochondrial Proteins and Transcripts in Human Cells. Smirnova A, Richert L, Smirnov A, Mély Y, Tarassov I.Methods Mol Biol. 2021;2277:157-173. doi: 10.1007/978-1-0716-1270-5_11. 

Super-resolution correlative light-electron microscopy using a click-chemistry approach for studying intracellular trafficking. Andrian T, Bakkum T, van Elsland DM, Bos E, Koster AJ, Albertazzi L, van Kasteren SI, Pujals S.Methods Cell Biol. 2021;162:303-331. doi: 10.1016/bs.mcb.2020.09.001. Epub 2020 Oct 16.