Our Cores
Confocal microscopy, NGS data analysis and DNA microarray analysis are among services provided by core laboratories supported by INBRE.
The University of Nebraska Medical Center and Creighton University Medical Center serve as hosts for these technologically advanced laboratories.
INBRE provides salary support for technical personnel, major equipment purchases and operating supplies. It also helps make the services of the core laboratories available to all investigators throughout the state.
Our cores are administrative,advanced microscopy, bioinformatics and genomics.
Administrative Core
Housed in the UNMC Department of Biochemistry and Molecular Biology, this core provides logistic support for all of the activities of the network. In addition, budgetary oversight, outreach programs and network coordination are administrative responsibilities.
Contact
- Email Paul Sorgen, PhD, director and principal investigator, or call 402-559-7557.
- Email Tami Houdesheldt, grant protocol associate, or call 402-559-3316.
Advanced Microscopy Core
The Nebraska INBRE Program has imaging cores on both the UNMC and Creighton University Medical Center campuses, serving users there, as well as those from UNL and regional institutions.
The UNMC Advanced Microscopy Core Facility houses state-of-the-art imaging technologies ranging from super-resolution (~ 0.120 um to 0.020 um) to microscopic (~ 0.300 um) to mesoscopic (~ 1 um) biomedical imaging. Learn about this facility's equipment.
The Creighton University Integrated Biomedical Imaging Facility specializes in advanced tools for cell and tissue imaging. In addition to microscopes, the facility provides users with access and support to advanced imaging analysis software that can allow users to glean critical information from experimental results. Learn about this facility's resources.
Bioinformatics Core
Our INBRE bioinformatics core is housed in the UNMC Bioinformatics and Systems Biology Core, which is designed to support and streamline multidisciplinary collaborative research in biomedical and health informatics. The purpose of this facility is to build a core infrastructure and provide expertise necessary to assist and support INBRE researchers in the network to develop software and technology solutions for the biosciences and healthcare.
Genomics Core
This UNMC Genomics Core Facility is a comprehensively equipped facility that provides genomic services to Nebraska's academic research community. The UNMC facility offers a variety of next-generation sequencing service applications, including RNAseq, miRNA, genomic resequencing and metagenomics. Single cell RNAseq and Nanostring Encounter gene expression panel profiling is also performed.
The presence of these cores currently supports the research of over 115 independent laboratories annually. These researchers are involved in a wide variety of scientific projects of both biomedical and basic science relevance.
Next Generation Sequencing
The DNA Sequencing Core operates the Illumina NovaSeq6000, NextSeq550, and MiSeq V2 next generation DNA sequencers. These three sequencers comprise a set of sequencers of varying capacity to enable a wide variety of applications and experiments that require different outputs. The NovaSeq is the largest capacity and has the best economy of scale. The NextSeq is for medium sized projects and the MiSeq is best for smaller genomes and 16s profiling.
Single Cell Genomics
The 10X Genomics instrument is designed to measure single cell RNAseq (scRNAseq) as well as single cell multiomics (scRNAseq coupled with same cell scATACseq) for experiments ranging from 2,000 to millions of cells. The core performs a great deal of scRNA utilizing the 10xGenomics system. We work with researchers to optimize cell suspensions, capture single cells and perform genome-wide transcription experiments on single cells.
Nanostring Encounter Expression Profiling
The Nanostring system is used for profiling gene-expression in targeted sets (up to 800 genes) of genes involved in specific biological pathways.
The core has observed tremendous growth throughout the last 20 years due to the support of the IDeA program grants. This funding has been critical to the development and support of the functional genomics infrastructure in Nebraska, and has contributed to the research efforts of many independent researchers.
The UNMC Genomics Core receives partial support from the National Institute for General Medical Science INBRE - P20GM103427-19 grant, as well as the Fred & Pamela Buffett Cancer Center Support Grant P30 CA036727 and the Nebraska Research Initiative.
Contact
- Email James Eudy, PhD, genomics core director, or call 402-559-3940.