Gurumurthy Channabasavaiah, Ph.D.

Professor

   

Department of Genetics, Cell Biology and Anatomy
985965 Nebraska Medical Center
Omaha, NE 68198-5965

402-559-8187
Email


Education:
MVSC (DVM) PhD, Indian Veterinary Research Institute 2000
Executive MBA, University of Nebraska at Omaha, 2012

Academic Appointments:
Professor, Genetics, Cell Biology and Anatomy.
Director, Mouse Genome Engineering Core Facility.

Research:
Research interests: genome editing and gene therapy technology development and mouse molecular genetics. While serving as the core director, and in collaboration with Dr. Masato Ohtsuka, Tokai University, Japan, our research work has developed many genome engineering and transgenic technologies. These include Easi-CRISPR GONAD, improved-GONAD, CRISPR-First;PITT-next, Pronulcear Injection based Targeted Transgenesis PITT and improved-PITT.
A short video about Easi-CRISPR and GONAD technologies is here 

Publications listed in PubMed

Selected Publications

  1. Gurumurthy CB*, Quadros RM, Ohtsuka M*. Prototype mouse models for researching SEND-based mRNA delivery and gene therapy. Nat Protoc. 2022 Aug 3. doi: 10.1038/s41596-022-00721-7. Epub ahead of print. PMID: 35922579.
  2. Gurumurthy CB*, Quadros RM, Richardson GP, Poluektova LY, Mansour SL, Ohtsuka M. Genetically modified mouse models to help fight COVID-19. Nat Protoc. 2020 Dec;15(12):3777-3787. doi: 10.1038/s41596-020-00403-2. PMCID: PMC7704938.
  3. Gurumurthy CB*, O'Brien AR, Quadros RM, Adams J Jr, Alcaide P, Ayabe S, Ballard J, Batra SK, Beauchamp MC, Becker KA, Bernas G, Brough D, Carrillo-Salinas F, Chan W, Chen H, Dawson R, DeMambro V, D'Hont J, Dibb KM, Eudy JD, Gan L, Gao J, Gonzales A, Guntur AR, Guo H, Harms DW, Harrington A, Hentges KE, Humphreys N, Imai S, Ishii H, Iwama M, Jonasch E, Karolak M, Keavney B, Khin NC, Konno M, Kotani Y, Kunihiro Y, Lakshmanan I, Larochelle C, Lawrence CB, Li L, Lindner V, Liu XD, Lopez-Castejon G, Loudon A, Lowe J, Jerome-Majewska LA, Matsusaka T, Miura H, Miyasaka Y, Morpurgo B, Motyl K, Nabeshima YI, Nakade K, Nakashiba T, Nakashima K, Obata Y, Ogiwara S, Ouellet M, Oxburgh L, Piltz S, Pinz I, Ponnusamy MP, Ray D, Redder RJ, Rosen CJ, Ross N, Ruhe MT, Ryzhova L, Salvador AM, Alam SS, Sedlacek R, Sharma K, Smith C, Staes K, Starrs L, Sugiyama F, Takahashi S, Tanaka T, Trafford AW, Uno Y, Vanhoutte L, Vanrockeghem F, Willis BJ, Wright CS, Yamauchi Y, Yi X, Yoshimi K, Zhang X, Zhang Y, Ohtsuka M, Das S, Garry DJ, Hochepied T, Thomas P, Parker-Thornburg J, Adamson AD, Yoshiki A, Schmouth JF, Golovko A, Thompson WR, Lloyd KCK, Wood JA, Cowan M, Mashimo T, Mizuno S, Zhu H, Kasparek P, Liaw L, Miano JM, Burgio G*. Reproducibility of CRISPR-Cas9 methods for generation of conditional mouse alleles: a multi-center evaluation, Genome Biol. 2019 Aug 26;20(1):171. doi: 10.1186/s13059-019-1776-2. PMCID: PMC6709553
  4. Gurumurthy CB*, Masahiro Sato, Ayaka Nakamura, Masafumi Inui, Natsuko Kawano, Md Atiqul Islam, Sanae Ogiwara, Shuji Takabayashi, Makoto Matsuyama, Shinichi Nakagawa, Hiromi Miura, Masato Ohtsuka*; Creating CRISPR-based germline genome engineered mice without ex vivo handling of zygotes by i-GONAD, Nature Protoc. 2019, doi.10.1038/s41596-019-0187-x, PMID: 31341289
  5. Gurumurthy CB, Lloyd KCK. Generating mouse models for biomedical research: technological advances. Dis Model Mech. 2019 Jan 8;12(1). doi: 10.1242/dmm.029462. Review. PubMed PMID: 30626588.
  6. Miura H, Quadros RM, Gurumurthy CB*, Ohtsuka M*. Easi-CRISPR for creating knock-in and conditional knockout mouse models using long ssDNA donors. Nat Protoc. 2018 Jan;13(1):195-215. doi: 10.1038/nprot.2017.153. PMID: 29266098.
  7. Quadros RM, Miura H, Harms DW, Akatsuka H, Sato T, Aida T, Redder R, Richardson GP, Inagaki Y, Sakai D, Buckley SM, Seshacharyulu P, Batra SK, Behlke MA, Zeiner SA, Jacobi AM, Izu Y, Thoreson WB, Urness LD, Mansour SL *, Ohtsuka M *, Gurumurthy CB*. Easi-CRISPR: a robust method for one-step generation of mice carrying conditional and insertion alleles using long ssDNA donors and CRISPR ribonucleoproteins. Genome Biol 2017;18: https://doi.org/10.1186/s13059-017-1220-4
  8. Jacobi AM, Rettig GR, Turk R, Collingwood MA, Zeiner SA, Quadros RM, Harms DW, Bonthuis PJ, Gregg C, Ohtsuka M, Gurumurthy CB*, Behlke MA*. Simplified CRISPR tools for fficient genome editing and streamlined protocols for their delivery into mammalian cells and mouse zygotes. 2017 May 15;121-122:16-28. doi: 10.1016/j.ymeth.2017.03.021. PubMed PMID: 28351759.
  9. Schilit SL, Ohtsuka M, Quadros RM, Gurumurthy CB*. Pronuclear Injection-Based Targeted Transgenesis. Curr Protoc Hum Genet. 2016 Oct 11;91:15.10.1-15.10.28. PMID: 27727435
  10. Gurumurthy CB*, Grati M, Ohtsuka M, Schilit SL, Quadros RM, Liu XZ. CRISPR: a versatile tool for both forward and reverse genetics research. Human Genetics. 2016; 135 (9) : 971-976, PMCID PMC5002245.
  11. Sato M, Ohtsuka M, Watanabe S, and Gurumurthy CB*: Nucleic Acids Delivery Methods for Genome Editing in Zygotes and Embryos: the Old, the New, and the Old-New. Biology Direct. 2016, 11(1):16. Review.
  12. Gurumurthy CB*, Takahashi G, Wada K, Miura H, Sato M, Ohtsuka M. GONAD: A Novel CRISPR/Cas9 Genome Editing Method that Does Not Require Ex Vivo Handling of Embryos. Curr Protoc Hum Genet. 2016 (88):15.8.1-15.8.12. PMID: 26724720
  13. Quadros R, Poluektova L, Gurumurthy CB*: Simple and Reliable Genotyping Protocol for Mouse PrkdcSCID J Immunol Methods. Volume 431, April 2016, Pages 60–62. PMID: 26851521.
  14. Quadros RM, Harms DW, Ohtsuka M, Gurumurthy CB*. Insertion of sequences at the original provirus integration site of mouse ROSA26 locus using the CRISPR/Cas9 system. FEBS Open Bio. 2015, 5: 191-197.
  15. Harms DW, Quadros RM, Seruggia D, Ohtsuka M, Takahashi G, Montoliu L, Gurumurthy CB*. Mouse Genome Editing Using the CRISPR/Cas System. Curr Protoc Hum Genet. 2014, Editor. Board Jonathan Haines Al 83, 15.7.1–15.7.27.