Advanced Microscopy
Some questions can only be answered by looking. Microscopy shows where molecules, cells, and structures actually sit—from detail finer than a wavelength of light, up to whole intact organs rendered in three dimensions.
The Advanced Microscopy Shared Resource gives researchers access to a full range of imaging systems, from super-resolution and confocal microscopes to whole-slide scanners and light sheet microscopes for intact tissue. Imaging specialists can help plan experiments, prepare samples, collect images, and analyze the results.
New Project Consultation
Are you starting a new project involving single or multiple Shared Resources? Or new to Fred & Pamela Buffett Cancer Center Shared Resources and want to know more about the services? Contact Dr. Kate Hyde to schedule a consultation or complete the consultation form.
Support at Every Stage
Consultation. Talk with imaging specialists before you start. Choosing the right instrument, preparing the sample, and picking labels are the decisions that most determine whether your experiment works.
Training and continued learning. Specialists train researchers to run most systems independently. The core maintains sample preparation guides, protocols, and recorded workshops for users at any experience level, runs regular workshops with instrument vendors, and keeps users informed as instruments and software are updated.
Sample preparation. The core can assist with preparations such as tissue clearing, a required step before most whole-tissue 3D imaging.
Imaging. Staff can collect your images for you, work alongside you, or set you up to run the instrument yourself. Access varies by instrument and is noted below.
Image analysis. Dedicated workstations with specialized software are available by request.
Data storage. Images are transferred to the core’s managed server, which helps meet NIH data management and sharing requirements.
Instruments
Instruments are grouped by the scale of what they can resolve, from the very small to whole intact tissues.
Pricing Benefits
Subsidized pricing is available to Fred & Pamela Buffett Cancer Center members. If you haven't received an email about subsidized pricing, please get in touch with cancer center administration to see if you qualify.
Contact: buffettcancercenter@unmc.edu
If you conduct cancer-related research at UNMC and are not yet a member of a Cancer Center research program, visit the Cancer Center Membership page to learn more about becoming a member.
Request Service
We use Stratocore PPMS, a centralized system that lets researchers request core facility services and manage scheduling and billing in one place. To request services, log in to PPMS and submit your project.
Request Service and View Pricing via Stratocore PPMS →
Citing Shared Resources
Researchers using the Cancer Center Shared Resources for publications, presentations, or other scholarly outputs are required to acknowledge the use of them. Proper acknowledgment helps support the continued availability and funding of these cores.
Please acknowledge the Cancer Center Support Grant (P30 CA036727) in all publications and abstracts that have used these services.
Suggested language: Research reported in this publication was supported by the Advanced Microscopy Shared Resource (RRID:SCR_022467), and the National Cancer Institute under award number P30 CA036727. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Cancer Institute.
Research Impact
/2
Researchers developed a faster method to make tissue transparent, then used multiphoton imaging to map pain-sensing nerve cells in three dimensions throughout intact nerve tissue.
/2
Second-harmonic generation imaging, which detects collagen without any staining, mapped how the dense, scar-like tissue surrounding pancreatic tumors differs between primary tumors and liver metastases, and how chemotherapy reshapes it.
Director
Heather Jensen-Smith, PhD
Director, Advanced Microscopy Shared Resource
Phone number: 402-559-6659