The UNMC Genomics Core Facility is a comprehensively equipped facility that provides genomic services to the greater academic research community in the State of Nebraska. The UNMC Genomics offers a variety of NGS service applications to include but not limited to, RNAseq, miRNA, genomic resequencing, and metagenomics. Single cell RNAseq and Nanostring Encounter gene expression panel profiling is also performed. The presence of these cores currently supports the research of over 115 independent laboratories annually. These researchers are involved in a wide variety of scientific projects of both biomedical and basic science relevance.
Next Generation Sequencing
The DNA Sequencing Core operates the Illumina NovaSeq6000, NextSeq550, and MiSeq V2 next generation DNA sequencers. These three sequencers comprise a set of sequencers of varying capacity to enable a wide variety of applications and experiments that require different outputs. The NovaSeq is the largest capacity and has the best economy of scale. The NextSeq is for medium sized projects and the MiSeq is best for smaller genomes and 16s profiling.
Single Cell Genomics
The 10X Genomics instrument is designed to measure single cell RNAseq (scRNAseq) as well as single cell multiomics (scRNAseq coupled with same cell scATACseq) for experiments ranging from 2,000 to millions of cells. The core performs a great deal of scRNA utilizing the 10xGenomics system. We work with researchers to optimize cell suspensions, capture single cells and perform genome-wide transcription experiments on single cells.
Nanostring Encounter Expression profiling
The Nanostring system is used for profiling gene-expression in targeted sets (up to 800 genes) of genes involved in specific biological pathways.
The core has observed tremendous growth throughout the last 20 years due to the support of the IDeA program grants. This funding has been critical to the development, the steady and sustained support of the functional genomics infrastructure in Nebraska, and as such has contributed to the research efforts of many independent researchers. The University of Nebraska DNA Sequencing Core receives partial support from the National Institute for General Medical Science (NIGMS) INBRE - P20GM103427-19 grant as well as The Fred & Pamela Buffett Cancer Center Support Grant - P30 CA036727, and the Nebraska Research Initiative.