The Nebraska INBRE Imaging Core Facility (NIICF) serves numerous users on the UNMC and CUMC campuses, as well as additional users from UNL and regional institutions.
The Advanced Microscopy Core Facility (AMCF) at UNMC houses state-of-the-art imaging technologies ranging from super-resolution (~ 0.120 um to 0.020 um) to microscopic (~ 0.300 um) to mesoscopic (~ 1 um) biomedical imaging. The Zeiss ELYRA PS.1 is an inverted microscope for super-resolution (SR) structured illumination microscopy (SIM) and single-molecule localization microscopy (SMLM) including, PhotoActivated Localization Microscopy (PALM) using photo-switchable/convertible fluorescent proteins, Total Internal Reflection Fluorescence (TIRF) and STochastic Optical Reconstruction Microscopy (STORM). The Zeiss 800 CLSM with Airyscan is an inverted microscope dramatically increasing conventional confocal image resolution to ~180 nm using Airyscan technology. The Zeiss 710 LSM is an inverted microscope supporting most basic imaging applications, multi-channel and spectral, co-localization, live cell, 3D, and time series imaging. The Zeiss Celldiscoverer 7 is a widefield imaging system for automated, time-lapse imaging of live samples. The Zeiss Axioscan 7 is a high-performance whole slide scanning system for fluorescence, brightfield, and polarization imaging. The Miltenyi Biotec Ultramicroscope II Light Sheet fluorescence microscope (LSFM) extends fluorescent imaging into true 3D, large-scale volumetric imaging of intact tissues, organs, and small organisms. The AMCF also houses several high-end data analysis workstations with premier image analysis software including HALO (Indica Labs) and IMARIS (Oxford Instruments) facilitating data rendering, analyses, and presentation options.
The Integrated Biomedical Imaging Facility at Creighton University is a multi-user core facility specializing in advanced live cell and tissue fluorescence imaging, and high content screening applications. Core technology includes the Leica TCS SP8 multiphoton laser scanning confocal microscope with 5 laser lines (405 nm, 488 nm, 514 nm, 552 nm, 633 nm) and a Spectra-Physics Mai Tai DeepSee tunable ultrafast laser (680 – 1040 nm) for multiphoton excitation. This upright microscope has a large, motorized stage sufficient for electrophysiology applications or live animal imaging. Ultrafast detectors and TCSPC electronics enable FLIM, FRET, and FCS fast dynamics studies, and spectral imaging detectors can separate and quantify fluorophores with overlapping emission. For faster confocal imaging, the facility has a Nikon TIE Inverted Yokagawa spinning disk confocal microscope capable of 100 frames/s full field of view. This microscope also has a temperature-controlled hydrated stage incubator for long term observations of living cells over multiple days. Finally, automated, high content screening applications can be performed either widefield (Molecular Devices IXM) or confocal (Molecular Devices IXU). Built in microfluidics allow automated delivery of reagents to selected wells in pre-determined amounts and at pre-determined times in a temperature and humidity-controlled environment. Finally, we have advanced imaging analysis software that can dramatically enhance the information content of your experimental results, including MetaXpress, Volocity, and Mathematica. Additional information regarding the IBIF is available at www.creighton.edu/researchservices/corefacilities/ibif.