Advanced Microscopy Core

This core facility serves numerous users on the UNMC and CUMC campuses, as well as additional users from UNL and other institutions. The UNMC campus houses two Zeiss LSM confocal microscopes (LSM 800 and LSM710) equipped with controlled temperature and CO2 environments for live image analysis. Each microscope has four laser lines and the LSM 800 has an Airyscan module that enhances confocal resolution by 1.8-fold. The facility has also a state-of-the-art dual-platform Zeiss ELYRA PS.1 super-resolution microscope, capable of 3D Structured Illumination Microscopy which improves resolution over that of typical confocal microscopy from ~300 nm in the XY axis to ~100 nm. This microscope is also capable of single-molecule imaging by using TIRF-based Photo Activatible Localization Microscopy (PALM) and direct Stochastic Optical Reconstitution Microscopy (dSTORM). These single molecule techniques can achieve 5-10 nm resolution under optimal conditions.

The Integrated Biomedical Imaging Facility at Creighton University is a multi-user core facility specializing in advanced live cell and tissue fluorescence imaging, and high content screening applications. Core technology includes the Leica TCS SP8 multiphoton laser scanning confocal microscope with 5 laser lines (405 nm, 488 nm, 514 nm, 552 nm, 633 nm) and a Spectra-Physics Mai Tai DeepSee tunable ultrafast laser (680 – 1040 nm) for multiphoton excitation.  This upright microscope has a large, motorized stage sufficient for electrophysiology applications or live animal imaging. Ultrafast detectors and TCSPC electronics enable FLIM, FRET, and FCS fast dynamics studies, and spectral imaging detectors can separate and quantify fluorophores with overlapping emission. For faster confocal imaging, the facility has a Nikon TIE Inverted Yokagawa spinning disk confocal microscope capable of 100 frames/s full field of view. This microscope also has a temperature-controlled hydrated stage incubator for long term observations of living cells over multiple days. Finally, automated, high content screening applications can be performed either widefield (Molecular Devices IXM) or confocal (Molecular Devices IXU). Built in microfluidics allow automated delivery of reagents to selected wells in pre-determined amounts and at pre-determined times in a temperature and humidity-controlled environment. Finally, we have advanced imaging analysis software that can dramatically enhance the information content of your experimental results, including MetaXpress, Volocity, and Mathematica. Additional information regarding the IBIF is available at www.creighton.edu/researchservices/corefacilities/ibif.