Department of Pharmaceutical Sciences
College of Pharmacy
University of Nebraska Medical Center
986025 Nebraska Medical Center
Omaha, NE 68198-6025
At the professional level, Dr. Alnouti lectures in the areas of Drug Metabolism and Pharmacokinetics in the "Introduction to Pharmaceutical Sciences" and "Pharmaceutical Sciences II & III" courses. At the graduate level, Dr. Alnouti provides lectures in "Advanced Pharmacokinetics and Pharmacodynamics" and the "Quantitative Pharmaceutical Analysis" courses.
Research in Dr. Alnouti's laboratory is involved in the multidisciplinary area of drug metabolism and pharmacokinetics (DMPK). His research focuses on the application of Bioanalytical Chemistry, in vitro and in vivo animal models to support ADMET (absorption, distribution, metabolism, excretion, and toxicity) and pharmacokinetic (PK) studies. This is a technique-driven enterprise; therefore expertise in liquid chromatography-mass spectrometry (LC-MS) is heavily used in his research.
Another area of interest in Dr. Alnouti's laboratory is the discovery of biomarkers for hepato-biliary diseases based on bile acid metabolism by sulfation, a phase II metabolic pathway.
- Huang J, Bathena SP, Alnouti Y. Metabolite profiling of praziquantel and its analogs during the analysis of in vitro metabolic stability using information-dependent acquisition on a hybrid triple quadrupole linear ion trap mass spectrometer. Drug MetabPharmacokinet. 2010; 25(5): 487-99. PMID: 20877135.
- Gautam N, Roy U, Balkundi S, Puligujja P, Guo D, Smith N, Liu XM, Lamberty B, Morsey B, Fox HS, McMillan J, Gendelman HE, Alnouti Y. Preclinical Pharmacokinetics and Tissue Distribution of Long-Acting Nanoformulated Antiretroviral Therapy. Antimicrob Agents Chemother. 2013 Jul; 57(7): 3110-20. PMID: 23612193. PMCID: PMC3697338. Featured by MDlinx in the area of Infectious Diseases on Dec 2013.
- Bathena SP, Mukherjee S, Olivera M, Alnouti Y. The profile of bile acids and their sulfate metabolites in human urine and serum. J Chromatogr B Analyt Technol Biomed Life Sci. 2013 Dec; 942-943:53-62. PMID: 24212143.
- Gautam N, Puligujja P, Balkundi S, Thakare R, Liu XM, Fox HS, McMillan J, Gendelman HE, Alnouti Y. Pharmacokinetics, Biodistribution, and Toxicity of Folic Acid-Coated Antiretroviral Nanoformulations. Antimicrob Agents Chemother. 2014 Oct 6. pii: AAC.04108-14. PMID: 25288084. PMCID: PMC4249580. Featured by MDlinx in the area of Infectious Diseases on Dec 2014. Featured by World Biomedical Frontiers in the area of Infection and Immunity.
- Bathena SP, Thakare R, Gautam N, Mukherjee S, Olivera M, Meza J, Alnouti Y. Urinary Bile Acids as Biomarkers for Liver Diseases I. Stability of the baseline profile in healthy subjects. Toxicol Sci. 2015 Feb, 143 (2): 296-307. PMID: 25344562.
- Bathena SP, Thakare R, Gautam N, Mukherjee S, Olivera M, Meza J, Alnouti Y. Urinary Bile Acids as Biomarkers for Liver Diseases II. Signature Profiles in Patients. Toxicol Sci. 2015 Feb, 143 (2): 308-18. PMID: 25344563.
- Nagsen Gautam, Rhishikesh Thakare, Sandeep Rana, Amarnath Natarajan, Yazen Alnouti. Irreversible Binding of an Anticancer Compound (BI-94) to Plasma Proteins. Xenobiotica. 2015 Oct, 45 (10): 858-73. PMID: 25869245. PMCID: PMC4553108.
- Thakare R, Chhonker YS, Gautam N, Alamoudi JA, Alnouti Y. Quantitative analysis of endogenous compounds. J Pharm Biomed Anal. 2016 Sep. 128:426-37. PMID: 27456759.
- Thakare R, Gao H1, Kosa RE, Bi YA, Varma MVS, Cerny MA, Sharma R, Kuhn M, Huang B, Liu Y, Yu A, Walker GS, Niosi M, Tremaine L, Alnouti Y, Rodrigues AD. Leveraging of Rifampicin-Dosed Cynomolgus Monkeys to Identify Bile Acid 3-O-Sulfate Conjugates as Potential Novel Biomarkers for Organic Anion-Transporting Polypeptides. Drug Metab Dispos. 2017 Jul;45(7):721-733. PMID: 28396527.
- Kindel TL, Krause C, Helm M3, McBride CL, Oleynikov D, Thakare R, Alamoudi J, Kothari V, Alnouti Y, Kohli R. Increased glycine-amidated hyocholic acid correlates to improved early weight loss after sleeve gastrectomy. Surg Endosc. 2017 Aug 4. [Epub ahead of print]. PMID: 28779240.