Luis A. Marky, Ph.D.
Luis A. Marky, Ph.D.

Professor

Department of Pharmaceutical Sciences
College of Pharmacy
University of Nebraska Medical Center
986025 Nebraska Medical Center
Omaha, NE 68198-6025
402-559-4628 (Office)
402-559-7870/5384 (Lab)
402-559-5673 (Fax)
Email

Teaching Activities:
At the professional level, Dr. Marky lectures in Medicinal Chemistry I where he is the course coordinator, Medicinal Chemistry II, and one elective course "Therapeutic Applications of Proteins and Nucleic Acids".  At the graduate level, Dr. Marky is the course coordinator for Quantitative Pharmaceutical Analysis and Biophysical Chemistry. In addition, he lectures in Advanced Medicinal Chemistry, and Macromolecular Structure and Function. Dr. Marky is a former Fulbright Scholar (Peru-98).

Research Activities/Interests:
The research interests of Dr. Marky are in the area of biophysical chemistry and focus on the molecular forces that control the conformational stability and flexibility of nucleic acids, including Okazaki fragments, hairpin loops, intramolecular triplexes, G-quadruplexes, C-quadruplexes, and three- & four-arm DNA junctions and their interaction with drugs. One objective is to improve the delivery of oligonucleotides into cells and drugs, by using natural and synthetic polymers, to block gene expression. His research group is also investigating the role of water on the conformational stability of biomolecules by examining sequence hydration effects in nucleic acids, drug-nucleic acid complexes, DNA covalent adducts, and protein-DNA interactions.

Recent Publications:

  1. Lee, H.-T.; Waters, L.; Olsen, C. M.; Khutsishvili, I.; Marky, L. A. “Probing the Temperature Unfolding of a Variety of DNA Secondary Structures Using the Fluorescence Properties of 2-aminopurine” Acta Chimica Slovenica, 59(3), 443–453 (2012).
  2. Zhang J, Reiling C, Reinecke JB, Prislan I, Marky LA, Sorgen PL, Naslavsky N, and Caplan S. “Rabankyrin-5 Interacts with EHD1 and Vps26 to Regulate Endocytic Trafficking and Retromer Function” Traffic, 13(5), 745-57 (2012).
  3. X-M Liu, Y. Zhang, F. Chen, I. Khutsishvili, E. V. Fehringer, L. A. Marky, K. W. Bayles, and D. Wang, “Prevention of Orthopedic Device-Associated Osteomyelitis Using Oxacillin-Containing Biomineral-Binding Liposomes.” Pharm Res., 29(11), 3169–3179 (2012).
  4. Khutsishvili, I.; Zhang, N.; Marky, L.A.; Crean, C.; Patel, D. J.; Geacintov, N. E. and Shafirovich, V. “Thermodynamic Profiles and Nuclear Magnetic Resonance Studies of Oligonucleotide Duplexes Containing Single Diastereomeric Spiroiminodihydantoin Lesions” Biochemistry, 52(8), 1354-1363 (2013). 
  5. Simone, P. D.; Struble, L.R; Kellezi, A.; Brown, C.A.; Grabow, C.E.; Khutsishvili. I.; Marky, L.A.; Pavlov, Y. I. and Borgstahl G.E.O. “The Human ITPA Polymorphic Variant P32T is Destabilized by the Unpacking of the Hydrophobic Core”, J. Struc. Biol, 182(3), 197–208 (2013).
  6. Gold, Barry; Stone, Michael P. and Marky, Luis A. “Looking for Waldo: A Potential Thermodynamic Signature to DNA Damage” Accounts of Chemical Research, 47(4), 1446-54 (2014).
  7. Khutsishvili, Irine; Johnson, Sarah E.; Reiling, Calliste; Prislan, Iztok; Lee, Hui-Ting and Marky, Luis A.; “Interaction of DNA Intramolecular Structures with Their Complementary Strands: A Thermodynamic Approach for the Control of Gene Expression” in Chemical Biology of Nucleic Acids: Fundamentals and Clinical Applications. Springer, 367-383 (2014).
  8. Reiling, Calliste and Marky, Luis A. “Contributions of the Loops on the Stability and Targeting of DNA Pseudoknots.” Biochemical Compounds 2,3 (2014).
  9. Prislan, Iztok; Lee, Hui-Ting.; Lee, Cynthia and Marky, Luis A. “Size of Internal Loop in DNA Hairpins Influences Targeting With Their Partially Complementary Strands” J. Physical Chemistry B. 119(1), 96-104 (2015).
  10. Reiling, Calliste, Khutsishvili, Irine; Huang, Kai and Marky, Luis A.; “Effect of Loop Length on DNA Pseudoknot Stability”. J. Physical Chemistry B, 119 (5), 1939–1946 (2015).