Don Ronning, PhD

Professorronning

Department of Pharmaceutical Sciences
College of Pharmacy
University of Nebraska Medical Center
986125 Nebraska Medical Center
Omaha, NE 68198-6125
402-559-7074 (Office)
402-559-5673(Fax)
Email

Research Activities/Interests: 

Dr. Ronning’s research interests are the discovery and development of anti-infective compounds with particular interest on compounds possessing efficacy for treating Tuberculosis. Tuberculosis causes more deaths worldwide than any other pathogen, yet the drugs used to treat this disease are In general, the lab’s aims are to understand the mechanistic details of protein function through X-ray crystallographic and enzyme kinetic studies, and to use that knowledge to identify inhibitors, assess mechanisms of inhibition, and detail the modes of interaction with the target protein. More specifically, we are interrogating enzymes within multiple Mycobacterium tuberculosis biosynthetic pathways that are either essential for bacterial viability or that are essential during infection of the human host. A new project in lab is focusing on the primase/helicase complex encoded by Human Cytomegalovirus, which is essential for replication of viral DNA.

Recent Publications:

  1. Nathan J. Holmes, Herbert W. Kavunja, Yong Yang, B. Dillon Vannest, Claudia N. Ramsey, Dana M. Gepford,Nicholas Banahene, Anne W. Poston, Brent F. Piligian, Donald R. Ronning, Anil K. Ojha, and Benjamin M. Swarts. A FRET-Based Fluorogenic Trehalose Dimycolate Analogue for Probing Mycomembrane-Remodeling Enzymes of Mycobacteria. (2019) ACS Omega DOI: 10.1021/acsomega.9b00130
  2. Sunayana Kapil, Cecile Petit, Victoria N. Drago, Donald R. Ronning, and Steven J. Sucheck. Synthesis and in Vitro Characterization of Trehalose-Based Inhibitors of Mycobacterial Trehalose 6-Phosphate Phosphatases. (2019) ChemBioChem DOI: 10.1002/cbic.201800551
  3. Abhishek Vartak, Christopher Goins, Vinicius Calado Nogueira de Moura, Celine M. Schreidah, Alexander D. Landgraf, Boren Lin, Jianyang Du, Mary Jackson, Donald R. Ronning, and Steven J. Sucheck. Biochemical and microbiological evaluation of N-aryl urea derivatives against mycobacteria and mycobacterial hydrolases. (2019) MedChemComm DOI: 10.1039/C9MD00122K
  4. Sunayana Kapil, Cecile Petit, Victoria N. Drago, Donald R. Ronning, and Steven J. Sucheck. Synthesis and in vitro characterization of trehalose‐based inhibitors of mycobacterial trehalose 6‐phosphate phosphatases. (2018) ChemBioChem doi.org/10.1002/cbic.201800551
  5. Christopher M. Goins, Thanuja Sudasinghe, Xiaofan Liu, Yanping Wang, George O'Doherty, Donald R. Ronning. Characterization of Tetrahydrolipstatin and Stereo-derivatives on the Inhibition of Essential Mycobacterium tuberculosis Lipid Esterases. (2018) Biochemistry 57(16):2383-2393. doi: 10.1021/acs.biochem.8b00152
  6. Christopher M. Goins, Steven Dajnowicz, Micholas D. Smith, Jerry M. Parks, and Donald R. Ronning. Mycolyltransferase from Mycobacterium tuberculosis in covalent complex with tetrahydrolipstatin provides insights into Antigen 85 catalysis. (2018) J Biol Chem. 293(10):3651-3662. doi: 10.1074/jbc.RA117.001681
  7. Michael T. Banco, Vidhi Mishra, Samantha C. Greeley, and Donald R. Ronning. Direct Detection of Products from S-adenosylmethionine-Dependent Enzymes using a Competitive Fluorescence Polarization Assay. (2018) Anal Chem. 90(3):1740-1747. doi: 10.1021/acs.analchem.7b03556
  8. Christopher M. Goins, Celine M. Schreidah, Steven Dajnowicz, Donald R. Ronning. Structural basis for lipid binding and mechanism of the Mycobacterium tuberculosis Rv3802 phospholipase. J Biol Chem. (2018) 293(4):1363-1372. doi: 10.1074/jbc.RA117.000240
  9. Christopher M. Goins, Steven J. Dajnowicz, Sandeep Thanna, Steven J. Sucheck, Jerry Matthew Parks, and Donald R Ronning. Exploring Covalent Allosteric Inhibition of Antigen 85C from Mycobacterium tuberculosis by Ebselen Derivatives. (2017) ACS Infectious Diseases. doi: 10.1021/acsinfecdis.7b00003
  10. Michael T Banco, Vidhi Mishra, Andreas Ostermann, Tobias E Schrader, Gary B Evans, Andrey Kovalevsky, and Donald R Ronning. Neutron structures of the Helicobacter pylori 5'-methylthioadenosine nucleosidase highlight proton sharing and protonation states. (2016) Proc Natl Acad Sci USA. 113(48):13756-61. doi.org/10.1073/pnas.1609718113
  11. Sandeep Thanna, Susan E Knudson, Anna Grzegorzewicz, Sunayana Kapil, Christopher M Goins, Donald R Ronning, Mary Jackson, Richard A Slayden, Steven J Sucheck. Synthesis and evaluation of new 2-aminothiophenes against Mycobacterium tuberculosis. (2016) Org Biomol Chem. 14(25):6119-33. doi: 10.1039/c6ob00821f
  12. Jared J. Lindenberger, Sri Kumar Veleti, Sandeep Thanna, Britney Wilson, Steven J. Sucheck, and Donald R. Ronning. Crystal structures of Mycobacterium tuberculosis GlgE and complexes with non-covalent inhibitors. (2015) Sci. Reports. 5:12830. doi: 10.1038/srep12830