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You can read about the usage of the program in more detail at the Mfold web server page
Usage is
mfold SEQ='file_name' with optional parameters: [ AUX='auxfile_name' ] [ RUN_TYPE=text (default) or html ] [ NA=RNA (default) or DNA ] [ LC=sequence type (default = linear) ] [ T=temperature (default = 37 deg C) ] [ P=percent (default = 5) ] [ NA_CONC=Na+ molar concentration (default = 1.0) ] [ MG_CONC=Mg++ molar concentration (default = 0.0) ] [ W=window parameter (default - set by sequence length) ] [ MAXBP=max base pair distance (default - no limit) ] [ MAX=maximum number of foldings to be computed (default 100) ] [ MAX_LP=maximum bulge/interior loop size (default 30) ] [ MAX_AS=maximum asymmetry of a bulge/interior loop (default 30) ] [ ANN=structure annotation type: none (default), p-num or ss-count ] [ MODE=structure display mode: auto (default), bases or lines ] [ LAB_FR=base numbering frequency ] [ ROT_ANG=structure rotation angle ] [ START=5' base # (default = 1)] [ STOP=3' base # (default = end) ] [ REUSE=NO/YES (default=NO) reuse existing .sav file ]