Duan Lab

Duan-Photo-2017.jpg

 

 

 

 Bin Duan, Ph.D. - Principal Investigator
DRC II 6035
UNMC, Omaha, NE 68198-5965
Phone: 402-559-9637
EMAIL

Research

The research goal of Dr. Duan’s lab (Biomaterials, Biofabrication and Biointerface Lab) is to create multi-scale complexity within engineered tissues to understand the fundamentals of cell-material interactions and mimic the native tissues for biomedical applications with a specific emphasis on tissue disease, tissue regeneration and stem cell bioengineering. Dr. Duan’s research involves: (i) utilizing biofabrication techniques to fabricate scaffolds with tailored micro- and macroscopic structures for specific tissue regeneration; (ii) developing 3D tissue constructs/organoids to investigate the pathogenesis of diseased tissue and examining potential therapeutic remedies; (iii) investigating the interaction of stem cells with defined microenvironments and developing materials-based techniques to control stem cell differentiation.

Selected Publications (*corresponding author)

  1. Duan B*. State-of-the-art review of 3D bioprinting for cardiovascular tissue engineering. Annals of Biomedical Engineering, 2017, 45, 195-209.
  2. Wang Y, Wu SH, Kuss MA, Streubel PN, Duan B*. Effects of hydroxyapatite and hypoxia on chondrogenesis and hypertrophy in 3D bioprinted ADMSC laden constructs. ACS Biomaterials Science and Engineering, 2017, 3: 826-835.
  3. Wu SH, Xu Ranjie, Duan B*, Jiang Peng. Three-dimensional hyaluronic acid hydrogel-based models for in vitro human iPSC-derived NPC culture and differentiation. Journal of Materials Chemistry B, 2017, 5: 3870-3878.
  4. Kuss MA, Harm R, Wu SH, Wang Y, Untrauer JB, Carlson MA, Duan B*. Short-term hypoxic preconditioning promotes prevascularization in 3D bioprinted bone constructs with stromal vascular fraction derived cells. RSC Advances. 2017, 7: 29312-29320.
  5. Wu SH, Wang Y, Streubel PN, Duan B*. Living nanofiber yarn-based woven biotextiles for tendon tissue engineering using cell tri-culture and mechanical stimulation. Acta Biomaterialia, 2017, 62: 102-115.

Complete List of Published Work in Google Scholar:  

https://scholar.google.com/citations?hl=en&user=9XjyCfwAAAAJ&view_op=list_works&sortby=pubdate

Patents

  1. Duan B, Kuss MA, Hollingsworth MA, Crawford, AJ. 3D printed abdominal viewing window with drug delivery. Pending.
  2. Duan B, Kuss MA, Kielian TL, Aldrich A. 3D printed scaffold for preventing recurrence of craniotomy-associated bacterial infection. Pending.

Grants

Active

  1. Mary & Dick Holland Regenerative Medicine Program Pilot Grant

07/2017-07/2019

Title: Cartilage Tissue Engineering and Regeneration

Role: PI

The goal of this project is to understand the mechanism of cartilage regeneration and engineer functional cartilage tissues using pig model.

  1. American Heart Association, Scientist Development Grant

07/2017-06/2020

Title: Bio-inspired engineered pediatric valve regulates MSC differentiation and MSC-monocyte interaction

Role: PI

The goal of this project is to mimic the environments of pulmonary valves in juvenile sheep with engineered valve constructs to understand how the age specific environments regulate MSC differentiation to valve cells

  1. Nebraska Stem Cell Research Project Grant

10/2017-09/2018

Role: PI

Title: MSC derived myelinating schwann cell for peripheral nerve regeneration

The goal of this project is to determine how the aligned nanofibrous topography and conductivity of nanofiber yarns affect human adipose derived mesenchymal stromal cell derived schwann cell (hADMSC-SC) myelination and human induced pluripotent stem cell-derived spinal cord neuronal progenitor cells (hiPSC-SCNPC) motor neuron differentiation and how the hADMSC-SC laden conductive nanofiber yarn-filled conduits affect large peripheral nerve defect regeneration.

  1. NIH R21 AI140026

06/2018-05/2020

Role: MPI

Title: Modeling chikungunya virus infection in a vascularized bone model

The goal of this project is to explore and elucidate the mechanisms underlying the bone pathology observed during CHIKV infection.

  1. NIH R01AR073225

06/2018-05/2023

Role: PI

Title: 3D bioprinting of biomimetic constructs for rotator cuff augmentation

The goal of this project is to combine biotextile and 3D bioprinting techniques to generate rotator cuff constructs with zonal cellular phenotypes and vascular patterns to promote infraspinatus rotator cuff healing in the rabbit model.

 6. Cancer and Smoking Disease Research Funds grant (LB506)

07/2018-06/2019

Role: MPI

Title: Validate 3D bioprinted hydrogels to discover hypoxia pathway inhibitors

The goal of this project is to utilize 3D bioprinted breast cancer cell model that has been developed and used to establish the role of hypoxia-inducible factor 1-alpha (HIF1α)-mediated hypoxia response that culminates in epithelial mesenchymal transition, to screen a kinase inhibitor library that includes members previously shown to inhibit the HIF-1 pathway.

Completed

  1. Nebraska Research Initiative Grant-University of Nebraska

10/2015-10/2017

Role: PI

The purpose of this grant is to equip the lab with advanced 3D bioprinter for tissue and organ bioprinting to enhance broader collaborations within University of Nebraska system.

  1. UNL and UNMC Sciences, Engineering, and Medicine Initiative

Role: MPI

07/2016-07/2018

Title: Engineered metabolically active brown adipose tissue from human iPSC to treat obesity and type 2 diabetes

The goal of this project is to generate engineering functional and implantable three-dimensional iPSC derived brown adipose tissues

Lab Members

Current Members:

Wen Shi (Post doc)

Mitchell Kuss (Technician)

Yunfan Kong (PhD candidate)           

Previous Members:

Ying Wang (Visiting Scholar)

Jainaha Srikumar (Summer Student)

Vaishnavi Ganesan (Summer Medical Student)

Blake Hass (Summer Student) 

Dianjun Qi (Visiting Scholar)

Wenhai Zhang (Visiting Surgeon)

Liang Wei (Visiting Scholar)

Shaohua Wu (Post doc)