Hyung Joon Kim, Ph.D. – Principal Investigator
DRC II 6034
UNMC, Omaha, NE 68198-5965
My laboratory has worked on measuring the pathophysiologically relevant molecular and cellular deficits in human brain diseases. Neuronal, glial cells, and their niche factors are essential components of the functional integrity of the brain in healthy normal and disease states. By manipulating cellular and niche components in an engineering dish, we have studied the mitochondria dysfunction in Alzheimer’s and Schizophrenia, axon pathfinding, dendritic morphogenesis of adult-born neurons, and glia-neuron interaction. The hippocampus-associated changes in the molecular signaling and circuit property have been commonly implicated in many neurodegenerative and psychiatric disorders including Alzheimer’s, Schizophrenia, and PTSD (post-traumatic stress disorder). However, there is lack of methods representing the human disease-relevant physiology in vitro. Using human patient’s induced pluripotent stem cells (hiPSCs) and engineering tissue scaffold, we aim to develop a microphysiological model system recapitulating the changes in the interaction between neuronal, glial, and neurovascular cells.
- To understand the human brain diseases through quantifying:
- Mitochondria dynamics, guidance, and myelination in axons.
- Differentiation and maturation of neural stem cell.
- Tools in the lab: Small-scale fabrication and neural stem cell biology.
Full list of publication (https://scholar.google.com/citations?user=oFQjVRoAAAAJ&hl=en)
- Han J., Kim H.J.*, Schafer S.T.*, Paquola A., Clemenson G.D., Toda T., Oh J., Pankonin A.R., Lee B.S., Sarkar A., Johnston S.T., Denli A.M., and Gage F.H., Functional implications of miR-19 in the migration of newborn neurons in the adult brain, Neuron, July 6 (2016) 91(1): 79-89, DOI: http://dx.doi.org/10.1016/j.neuron.2016.05.034 (*equal contribution)
- Kim H.J.*, Denli A.M.*, Wright R., Baul T.D., Clemenson G.D., Morcos A.S., Zhao C., Shafer S., Gage F.H., and Kagalwala M.N., REST regulates non- cell autonomous neuronal differentiation and maturation of neural progenitor cells via secretogranin II, J. Neurosci, November 4 (2015) 35(44):14872–14884, DOI:10.1523/JNEUROSCI.4286-14.2015 (*equal contribution)
- Kerman B.E., Kim H.J., Mei A., Padmanabhan K., Georges S., Jones M., Fitzpatrick J., Jappelli R., Chandross K., August P., and Gage F.H. (2015), In vitro myelination using embryonic stem cells, Development, 142, 1-13 doi:10.1242/dev.116517.
- Deshmukh, V. A., Tardif, V., Lyssiotis, C. A., Green, C. C., Kerman, B., Kim, H. J., Padmanabhan, K., Swoboda, J. G., Ahmad, I., Kondo, T., Gage, F. H., Theofilopoulos, A. N., Lawson, B. R., Schultz, P. G., and Lairson, L. L. (2013) A regenerative approach to the treatment of multiple sclerosis, Nature 502, 327-332.
- Butko, M. T., Yang, J., Geng, Y., Kim, H. J., Jeon, N. L., Shu, X., Mackey, M. R., Ellisman, M. H., Tsien, R. Y., and Lin, M. Z. (2012) Fluorescent and photo-oxidizing TimeSTAMP tags track protein fates in light and electron microscopy, Nat Neurosci. 15, 1742-1751.
- Kim, H. J., Park, J. W., Byun, J. H., Poon, W. W., Cotman, C. W., Fowlkes, C. C., and Jeon, N. L. (2012) Quantitative Analysis of Axonal Transport by Using Compartmentalized and Surface Micropatterned Culture of Neurons, ACS Chemical Neuroscience 3, 433-438.
- Kim, H. J., Park, J. W., Byun, J. H., Vahidi, B., Rhee, S. W., and Jeon, N. L. (2012) Integrated Microfluidics Platforms for Investigating Injury and Regeneration of CNS Axons, Ann Biomed Eng.
- Cohen, M. S., Bas Orth, C., Kim, H. J., Jeon, N. L., and Jaffrey, S. R. (2011) Neurotrophin-mediated dendrite-to-nucleus signaling revealed by microfluidic compartmentalization of dendrites, Proc Natl Acad Sci U S A 108, 11246-11251.
- Hengst, U., Deglincerti, A., Kim, H. J., Jeon, N. L., and Jaffrey, S. R. (2009) Axonal elongation triggered by stimulus-induced local translation of a polarity complex protein, Nat Cell Biol 11, 1024-1030.
- Nebraska Stem Cell Research Project Grant
Title: MSC derived myelinating Schwann cell for peripheral nerve regeneration.
Role: Co-Investigator (10/2017-06/2018)
Summary: Collaborating with Duan lab, we pursue to establish an engineered platform for promoting myelination of human adipose-derived mesenchymal stromal cell-derived Schwann cell (hADMSC-SC) and human induced pluripotent stem cell-derived spinal cord neuronal progenitor cells (hiPSC-SCNPC) motor neurons.
- Mary & Dick Holland Regenerative Medicine Program Pilot Grant
Title: Develop human neural progenitor-based in vitro models for mild traumatic brain injury.
Role: PI (01/2018-12/2018)
Summary: The goal of this project is to define an innate regenerative mechanism to limit longer-term injury after mild TBI.
Inquiry for an opening should be addressed to Hyung Joon Kim (email@example.com).