Assistant Professor, Biochemistry and Molecular Biology
Phone: 402-559-7566 (Lab)
PhD, University of Paris-Saclay, France, 2003
The main goal of my ongoing research projects is to understand the structural and functional mechanisms regulating gap junctions using a multidisciplinary approach. A major effort of mine is to investigate the regulatory mechanisms controlling the most abundant cardiac gap junction protein, connexin43 (Cx43), by focusing on intra- and intermolecular interactions that are involved in diseases and ischemic events. More specifically, ubiquitination and phosphorylation of Cx43 have been shown to play important roles in regulating the turnover and consequently the gap junction communication. We believe that a better understanding of the structural basis of connexins regulation will lead to the development of new strategies to modulate gap junction communication affected in the disease state.
Spagnol, G.*, Kieken, F.*, Kopanic, J.L., Li, H., Zach, S., Stauch, K.L., Grosely, R., Sorgen, P.L. (2016) Structural Studies of the Nedd4 WW Domains and their Selectivity for the Cx43 Carboxyl-terminus. J. Biol. Chem. 291, 7637-50
Ambrosi, C., Ren, C., Spagnol, G., Cavin, G., Cone, A., Grintsevich, E.E., Sosinsky, G.E., Sorgen, P.L. (2016) Connexin43 Forms Supramolecular Complexes through Non-Overlapping Binding Sites for Drebrin, Tubulin, and ZO-1. PLoS One. 11, e0157073.
Spagnol, G.*, Al-Mugotir, M.*, Kopanic, J.L., Zach, S., Li, H., Trease, A.J., Stauch, K.L., Grosely, R., Cervantes, M., Sorgen, P.L. (2016) Secondary structural analysis of the carboxyl-terminal domain from different connexin isoforms. Biopolymers. 105, 143-62
Li, H., Spagnol, G., Naslavsky, N., Caplan, S., Sorgen, P.L. (2014) TC-PTP directly interacts with connexin43 to regulate gap junction intercellular communication. J. Cell Sci. 127, 3269-79
Spagnol, G., Sorgen, P.L., Spray, D.C. (2014) Structural order in Pannexin 1 cytoplasmic domains. Channels 8,157-66