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Dr. Chand's Laboratory

Subhash Chand, PhD

Research Statement

HIV infection increases the chances of heart failure by twofold. Even in people living with HIV on combinational antiretroviral therapy, HIV replicates and releases HIV proteins in the circulation, causing systemic inflammation. Thus, people living with HIV have chronic inflammation, and their immune system is continuously activated.

Extracellular vesicles are critical in intercellular communication, inflammation, and disease progression. EVs carry inflammatory cytokines, proteins, and miRNAs in their cargo. Inflammation and HF are strongly interconnected and mutually reinforce each other. Thus, we sought to establish a connection between chronic inflammation to HF via EVs in HIV infection.

Stem cell-based therapeutics have shown that they are effective in treating various diseases. Deeper analyses of stem cell-based therapeutics revealed that the beneficial treatment impacts are mainly due to their paracrine influence via EVs. Also, mesenchymal stem cell-derived EVs have shown that they can repair injured myocardium and improve cardiac function. We plan to tag the MSC-EVs with a cardiac hoping peptide for their efficient delivery to the myocardium. This innovative idea will elucidate the therapeutic potential of MSC-EVs and the targeting ability of CHP to improve HF symptoms in PLWH.  

Faculty

Subhash Chand, PhD

Instructor, UNMC Department of Anesthesiology
Research Faculty

Subhash Chand, PhD
Research Interests
  • HIV infection, substance abuse, and related cardiovascular comorbidities.
  • Impact of inflammation and extracellular vesicles in HIV infection-associated comorbidities.
  • Modulation in endocannabinoid system in HIV infection and substance use disorders.
Funding

Bridge Funding Award ($76,500) - 01/01/2023 - 12/31/2023

Lieberman Research Award ($50,000) - 05/03/2022 - 12/31/2023

Nebraska Center for Nanomedicine Core ($5,000) - 08/01/2022 - 09/30/2023

Mini Sabbatical (MSab) Funding ($2,500) 03/01/2022 - 09/30/2022

Select Publications

 

  • Chand S, DeMarino C, Gowen A, Cowen M, Al-Sharif S, Kashanchi F, Yelamanchili SV, "Methamphetamine Induces the Release of Proadhesive Extracellular Vesicles and Promotes Syncytia Formation: A Potential Role in HIV-1 Neuropathogenesis" (Viruses, 2022) Impact Factor = 4.7. PMID: 35336957; PMCID: PMC8950763; DOI: 3390/v14030550
  • Chand S, Gowen A, Savine M, Moore D, Clark A, Huynh W, Wu N, Odegaard K, Weyrich L, Bevins RA, Fox HS, Pendyala G, Yelamanchili SV, "A comprehensive study to delineate the role of an extracellular vesicle-associated microRNA-29a in chronic methamphetamine use disorder Journal of Extracellular Vesicles" (Journal of Extracellular Vesicles, 2021) Impact Factor = 16.0. PMID: 34913274; PMCID: PMC8674191; DOI: 1002/jev2.12177
  • Chand S, Jo A, Vellichirammal NN, Gowen A, Guda C, Schaal V, Odegaard K, Lee H, Pendyala G, Yelamanchili SV, "Comprehensive characterization of organ-specific extracellular vesicles protein markers: Implications for preclinical and clinical applications" (ACS Applied Nano Materials, 2020) Impact Factor = 5.9. PMID: 33385108 PMCID: PMC7771629 DOI: 1021/acsanm.0c01654.
  • Ananthaswamy N, Fang Q, AlSalmi W, Jain S, Chen Z, Klose T, Sun Y, Liu Y, Mahalingam M, Chand S, Tovanabutra S, Robb ML, Rossmann MG, Rao VB, "A sequestered fusion peptide in the structure of an HIV-1 transmitted founder envelope trimer" (Nature Communications, 2019). Impact Factor = 16.6. PMID: 30787293; PMCID: PMC6382815; DOI: 1038/s41467-019-08825-7.
  • Chand S, Messina EL, AlSalmi W, Ananthaswamy N, Gao G, Uritskiy G, Padilla-Sanchez V, Mahalingam M, Peachman KK, Robb ML, Rao M, Rao VB, "Glycosylation and oligomeric state of envelope protein might influence HIV-1 virion capture by α4β7 integrin" (Virology, 2017). Impact Factor = 3.7. PMID: 28577856. PMCID: PMC5526109. DOI: 1016/j.virol.2017.05.016