University of Nebraska Medical Center

Electron Paramagnetic Resonance Spectroscopy

Bruker E-Scan Table Top EPR spectrometer

Electron Paramagnetic Resonance Spectroscopy, also known as electron spin resonance or EPR, is based on absorption of microwave energy by free radicals or paramagnetic species in the presence of an external electromagnetic field, resulting in the transition of electron spin states.

This core facility within the UNMC Department of Cellular and Integrative Physiology houses a Bruker E-Scan Table Top EPR spectrometer.

In combination with EPR-detectable, radical-sensitive cyclic hydroxylamines – often referred to as “spin probes” – the E-Scan can be and has been used to measure levels of free radicals (e.g. superoxide, hydroxyl radical) in various biological samples including cultured cells, culture media, blood, and isolated tissue.


Vice Chair of Education, UNMC Department of Cellular and Integrative Physiology
Director, Interdisciplinary Graduate Program in Biomedical Sciences
Director, Master of Science in Medical Physiology Program
Director, Electron Paramagnetic Resonance Spectroscopy Core and the Physiological Environment Research Core Facility


Matthew C. Zimmerman, PhD