University of Nebraska Medical Center

Physiological Environment Research

A HypOxystation H135 controlled environment chamber and IncuCyte S3 Live-Cell Analysis System.

A major limitation to modern day tissue and cell culture is the lack of control of environmental variables, such as oxygen, carbon dioxide and humidity, to mimic exact in vivo conditions. As such, cells are often exposed to atmospheric and non-physiological levels of these surroundings, which ultimately confounds data analysis and interpretation.

The Physiological Environment Research Facility of the UNMC Department of Cellular & Integrative Physiology works to limit these artifactual environmental variables by the use of tightly-regulated atmospheric conditions for tissue and cell culture-based experiments.

The core consists of this state-of-the-art piece of technology:

  • HypOxystation H135 controlled environment chamber.

The H135 chamber is a fully-enclosed glove-access workstation/incubator with the ability to alter oxygen (0-21%), carbon dioxide (0-15%), nitrogen (67-100%), humidity (0-90%), and temperature (12-45°C).


The facility operates on a fee-for-service basis and requires technician involvement due to extensive training required for the piece of equipment.


Vice Chair of Education, UNMC Department of Cellular and Integrative Physiology
Director, Interdisciplinary Graduate Program in Biomedical Sciences
Director, Master of Science in Medical Physiology Program
Director, Electron Paramagnetic Resonance Spectroscopy Core and the Physiological Environment Research Core Facility


Matthew C. Zimmerman, PhD