Physiological Environment Research

A major limitation to modern day tissue and cell culture is the lack of control of environmental variables, such as oxygen, carbon dioxide and humidity, to mimic exact in vivo conditions. As such, cells are often exposed to atmospheric and non-physiological levels of these surroundings, which ultimately confounds data analysis and interpretation.

The Physiological Environment Research Facility of the UNMC Department of Cellular & Integrative Physiology works to limit these artifactual environmental variables by the use of tightly-regulated atmospheric conditions for tissue and cell culture-based experiments.

The core consists of this state-of-the-art piece of technology:

• HypOxystation H135 controlled environment chamber.

The H135 chamber is a fully-enclosed glove-access workstation/incubator with the ability to alter oxygen (0-21%), carbon dioxide (0-15%), nitrogen (67-100%), humidity (0-90%), and temperature (12-45°C).

Fees

The facility operates on a fee-for-service basis and requires technician involvement due to extensive training required for the piece of equipment.