The Human Butyrylcholinesterase Production Facility is capable of producing human butyrylcholinesterase (BChE) in gram quantities from Cohn IV-4 paste (for research purposes only). Production uses a modification of the process described by Saxena et.al [1], employing hupresin-Sepharose instead of procainamide-Sepharose as the affinity resin. Process modifications were designed by Dr. Oksana Lockridge (publication pending).
Description
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Standard Activity Assay Conditions |
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Availability and Price
Human butyrylcholinesterase is available for purchase by qualified research institutions or companies. |
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Catalogue number: |
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NP06276 |
1 milligram vial |
Available to ship 5/1/2016 |
Call or e-mail for quote |
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For all questions related to this service or to obtain BChE contact:
Larry Schopfer
Eppley Institute
University of Nebraska Medical Center
Omaha, NE 68198-5950
phone 1-402-559-6305
fax 1-402-559-7838
lmschopf@unmc.edu
We are open to collaborations with academic laboratories as a mechanism for reducing cost. For discussions on collaboration, please contact Dr. Oksana Lockridge.
Production Facility Directors
Dr. Steven Hinrichs |
Dr. Oksana Lockridge |
References
1 Saxena, Tipparaju, Luo & Doctor; Pilot-scale production of human serum butyrylcholinesterase suitable for use as a bioscavenger against nerve agent toxicity; Process Biochemistry 45, 1313-1318 (2010)
Certificate of Analysis
Human butyrylcholinesterase in phosphate buffered saline
25 mg in 1 mL per vial, under nitrogen
Lot #: October 2015
Activity 12,500 Units/mL
measured in 0.1 M potassium phosphate pH 7, 1 mM butyrylthiocholine, 25˚C
Filter-sterilized through 0.22 µm membrane
MW 340,000; tetramer of 4 identical subunits
Purified from Cohn fraction IV-4 paste
Storage conditions: 4˚C
Endotoxin: 2 EU/mL
Manufactured by the University of Nebraska Medical Center
Steven H. Hinrichs, MD Oksana Lockridge, PhD University of Nebraska Medical Center University of Nebraska Medical Center Omaha, NE 68198-5900 Omaha, NE 68198-5950 shinrich@unmc.edu olockrid@unmc.edu |