Molecular Diagnostics

The Molecular Diagnostics Laboratory is a full-service clinical laboratory offering tests in all areas of molecular diagnostics, including infectious disease, inherited conditions, molecular hematology, molecular oncology, and pharmacogenetics. The comprehensive test menu is continually being updated with the newest diagnostic tests to help clinicians and pathologists provide the most appropriate and state-of-the-art care. Multiple full-time faculty members are involved with clinical case reporting and test validation.  Allison Cushman-Vokoun, M.D., Ph.D., and James Wisecarver, M.D., Ph.D., are the laboratory's medical directors and are board certified in Molecular Genetic Pathology with expertise in solid tumors and inherited diseases. Cathy Gebhart, Ph.D., the technical director, has expertise in molecular infectious disease. Julia Bridge, M.D., subspecialty boarded in Clinical Cytogenetics, is an expert in molecular sarcoma pathology as well as other solid tumors. Timothy Greiner, M.D., is an expert in molecular hematopathology

For medical or technical questions regarding the Molecular Diagnostics laboratory, please contact Dr. Cushman-Vokoun at 402-559-4186.

Medical Directors Director Histocompatibility Laboratory Manager
Allison Cushman-Vokoun, M.D., Ph.D.
James Wisecarver, M.D., Ph.D.
Catherine Gebhart, Ph.D. 
Phone: 402-559-7660 or E-mail
Jill Branson, MS
Phone: 402-559-7611 or E-mail
Histocompatibility: 402-559-7630 / Pager 402-888-1316

Molecular Oncology: 402-559-7745 / 402-559-8556

T –cell AHG Cytotoxic Crossmatch
B-cell Cytotoxic Crossmatch
Auto Lymphocyte Crossmatch
T and B cell Flow Cytometry Crossmatch
HLA Antibody Detection and Identification
HLA Class I &II Molecular Typing (low/intermediate/high)
Chimerism testing (unsep, CD3, CD33)

Ewing's Sarcoma, t (11;22)
Rhabdomyosarcoma, t (2;13)
Synovial Sarcoma, t(X;18)
EGFR Mutations
KRAS mutation detection (codon 12, 13 and 61)
BRAF mutation detection c.1799T>A (p.V600E)
Microsatellite Instability (MSI) Analysis
50 Gene Cancer Panel
Colorectal cancer mutation panel (includes KRAS, NRAS,  BRAF, PIK3CA,  HRAS)
Lung Mutation Panel (includes EGFR, BRAF, KRAS, NRAS, ERBB2, ERBB4, PIK3CA, PTEN, and AKT1
IDH1 and IDH2 mutation panel
Melanoma mutation panel (includes BRAF, KIT, NRAS, GNA11, GNAQ, and HRAS
GIST Mutation Panel (includes KIT, PDGFR, and BRAF
GNAS mutation detection
CTNNB1 mutation detection
B-cell Clonality
Bcr/Abl, t(9;22) (p190 and p210)
Bcr/Abl, t(9;22) quant (p210)
JAK2, p.V617F Mutation Assay
FLT3 3 ITD Mutation Detection
MPL Mutation Detection
NPM1 Mutation
T-cell Clonality
CALR mutation testing

Disease Association Testing: 402-559-7630

Birdshot Chorioretinopathy (HLA-A*29:01 and A*29:02)
Carbamazepine A*31:01-European/B*15:02-Asian
Type 1 Autoimmune Hepatitis (HLA- DRB1*03 and DRB1*04)
Autoimmune Thyroid Disease (HLA-DRB1*03 and DRB1*05)
Ankylosing Spondylitis B*27
Abacavir Hypersensitivity (HLA-B*57:01)
Allopurinol Hypersensitivity (HLA-B*58:01)
Behcet's (HLA-B*51)
Celiac Disease DQ2, DQ8
Narcolepsy DQB1*06:02
Vogt-Koyanagi-Harada Syndrome (HLA-DRB1*04:05)
Myelodysplastic Syndrome (HLA-DRB1*15)
Psoriatic Arthritis (HLA-C*06)
Rheumatoid Arthritis DRB1
Sjogren Syndrome
Systemic Lupus Erythematosus  (HLA-DRB1*15:01 and DRB1*03:01)
Other- Indicate antigen/allele association and disease

Genetics: 402-559-7744 Infectious Disease: 402-559-7744

Serpina-1 Z (p.E342K) and S (p.E264V) mutation
Factor II - Prothrombin (G20210A)
Factor V - Leiden (G1691A)
MTHFR Mutations (C677T & A1298C)
Fragile X- PCR analysis
Hemochromatosis (G845A & C187G)
Cystic Fibrosis (39 Mutations)

Adenovirus DNA Detection
BK Virus DNA Detection and Quantitation
Bordetella Pertussis DNA Detection
Cytomegalovirus (CMV) DNA Detection & Quantification
Enterovirus RNA Detection
Epstein-Barr Virus (EBV) DNA Detection & Quantitation
Hepatitis B Virus (HBV) Quantitation
Hepatitis C Virus (HCV) Quantitation and Genotyping
Herpes Simplex 1 & 2 DNA Detection
HIV Quantitation
Human Herpes Virus 6 (HHV6) DNA Detection
JC Virus DNA Detection
Norwalk Virus RNA Detection & Genotype
Parvovirus B19 DNA Detection
Varicella Zoster Virus (VZV) DNA Detection
Herpes Virus Panel (CMV, HSV, EBV, VZV, HHV-6)