Flow Cytometry Research Facility

Announcement - FCRF COVID-19 Policy

The Flow Cytometry Research Facility is open for services. We will remain open for research per UNMC guidelines, however at times we will have limited staffing.  We ask that our users follow BSL2 protocols in the facility, such as wearing gloves and wiping down work spaces with bleach wipes to support the effort to limit the spread of COVID-19. Users must practice social distancing during acquisition, with no more than 1 user per room. Per UNMC guidelines, please do not come to the facility if you have symptoms or a known exposure.  Closures and updates will be announcement on our website, via our User Group Email, and the VCR COVID-19 blog.  We are still offering out of hours independent acquisition, contact us for training if needed.



The Flow Cytometry Research Facility is a shared resources laboratory at UNMC, serving researchers and surrounding institutions by providing a central location for flow cytometry instrumentation and education. We offer a number of services to both internal and external scientists.

What is Flow Cytometry?

Flow cytometry is a rapid, multiparametric, single cell analysis technology that can look at phenotypic and functional characteristics of large numbers of cells in suspension. Below is short video on the basic concepts of flow cytometry. 
Click on the icon to launch the video.

 Click to Play Video

 Play Video

News and Class Schedule

Flow Cytometry Bootcamp

This summer, the Flow Cytometry Research Facility will be hosting a few sessions of  Flow Cytometry Boot Camp!  These will be week long classes, scheduled for approximately 1 hour each morning.  Instrument QC, fluidics, startup & shutdown, experimental setup and troubleshooting will be covered.  This class is intended for users who are already trained and experienced in acquiring their own samples on the LSRII instruments, but who want to learn more and increase their expertise in flow cytometry. 
Email vbsmith@unmc.edu for more information or to join the waitlist!

Introduction to Flow Cytometry Classes

New users need to complete the Practical Flow Cytometry class, offered at no charge to  University of Nebraska users by the facility (class fee for external users is posted under our rate schedule.) This scheduled class is 3 hours, and covers principles of flow cytometry technology, application, and using the facility. Space is limited, to register for this class call 402-559-6267 or email FCRF@unmc.edu. Class schedule is below.




2/2/2024 9:00 - 12:00 TBD
3/6/2024 9:00 - 12:00 TBD
4/3/2024 9:00 - 12:00 TBD
6/4/2024 9:00 - 12:00 TBD
7/2/2024 9:00 - 12:00 TBD

Subscribe to our email list here.

Follow us on Twitter @FlowUNMC for up to date news on the facility and for general flow cytometry information

Each month, we feature a Flow Cytometry Application of the Month on our Facebook Page.  Follow us on Facebook to learn tips & tricks to help you get the best data possible!

Acknowledgement and Support

When publishing data that has been generated at the facility or with support of facility staff, this must be acknowledged.

The UNMC Flow Cytometry Research Facility is administrated through the Office of the Vice Chancellor for Research and supported by state funds from the Nebraska Research Initiative (NRI) and The Fred and Pamela Buffett Cancer Center's National Cancer Institute Cancer Support Grant.  Major instrumentation has been provided by the Office of the Vice Chancellor for Research, The University of Nebraska Foundation, the Nebraska Banker's Fund, and by the NIH-NCRR Shared Instrument Program