Services & Rates

Click on each section below to display current rates for these services.

Minimum booking: 30 minutes analysis/60 minutes sorting
Booking increments: 15 minutes
Cancellations: Must be made with at least one working day notice.

We offer a number of supplemental services at the facility, including assisted data analysis (for beginners, complex analyses, and publication-quality data presentation); AutoMACS magnetic cell separation and enrichment; Countess automated hemocytometer; and consultations on all aspects of flow cytometry application.

*These rates are effective July 1st 2016.

New Users  

New users need to complete the Practical Flow Cytometry class, offered free by the facility. This regularly scheduled class is 3 hours, and covers principles of flow cytometry technology, application, and using the facility. Space is limited, to register for this class call 402-559-6299 or email Craig Semerad. View the class schedule here.

Flow Cytometry Analysis  
The Flow Cytometry Research Facility maintains several different flow cytometers for acquisition of samples. Capabilities of these instruments can be found on the Equipment page here. These cytometers are available 24/7 for trained users, and during regular operating hours for assisted acquisition.

Service

Rate / hr*

Unassisted acquisition

$37

Assisted acquisition

$63

Training fee

$37

Out of hours training

$37

Cell Sorting  
If you are after physically isolating sub-populations of cells, such as after a GFP transfection, or stem cell isolation, then you will need to use a cell sorter for high purity.  There are two multi-laser flow cytometric cell sorters in the facility and an AutoMACS magnetic particle separator for cell separation and enrichment.  Prices for Cell Sorting are below:

Service

Rate / hr*

Cell sorting

$52

 

+ $63 set-up fee

Independent Cell Sorting Training Fee

$750/3-part training (pre-requisites required, minimum training fee)

AutoMACS

$5/separation
+ $18/set-up fee
+ $75/column (2-week lifespan, cost shared amongst users)

Software  
We have numerous software packages at the facility to analyze flow cytometry data. These are available to users at no charge, including ModFit LT, BD FACSDiva v6, Cell Quest Pro, and FlowJo vX, from the facility workstations.

Additionally, we offer University of Nebraska colleagues the ability to sign up to our FlowJo site license, allowing access to this analysis software from any computer. There is a quarterly fee of $70 per computer, based on usage. To sign up to this site license please contact Craig Semerad.

External User Rates  
We offer all services to non-University of Nebraska affiliated groups but not at the subsidized rates. The table below lists all major services and the associated fee for external users. 

Custom services can be requested, please contact Craig Semerad for quotation or more information.

Service Rate/hr
Assisted Acquisition $126
Cell Sorting $104
  +$126 set-up fee
Unassisted Data Analysis $74
Assisted Data Analysis $126
AutoMACS $10/separation
  + $36 set-up fee
  + $150/column (2-week lifespan, cost shared amongst users)
Countess $5/slide
Consultation $74
Practical Flow Cytometry Class $210/person
Unclogging Fee $70
Other Services  

We offer a number of supplemental services at the facility, including assisted data analysis (for beginners, complex analyses, and publication-quality data presentation); Countess automated hemocytometer; and consultations on all aspects of flow cytometry application. 

Services Rate
Unassisted data analysis no charge
Assisted data analysis $63/hour
Countess $2.50/slide
Consultation no charge
Unclogging Fee $35