Pricing

Prices for various services provided by the UNMC Genomics Core Facility are included for researchers within the University of Nebraska system (UN System), as well as researchers from other academic institutions (Outside Users).

The following prices are effective as of July 1, 2020.


LIBRARY CONSTRUCTION PRICES

SERVICE PRICES
  UN System Outside Users
RNA Sequencing Libraries Per Sample
mRNA Sequencing Libraries $ 143.00 - $ 153.00 $ 179.00 - $ 192.00
miRNA Seq (NextFlexV3) BIOO $265.00* $331.25*
Total RNA Seq Library $215.00 $269.00
ClontechSmartSeqV4 + Nextera XT $370.00 $462.50
*based on a minimum of (3) samples  
DNA Sequencing Libraries Per Sample
Nextera Flex $140.00 $168.00
Metagenomics 16s V3V4 $15.00 $18.75

NEXT GENERATION SEQUENCING PRICES

INSTRUMENT PRICES
  UN System Outside Users
NovaSeq 6000 (price per flow cell)
NovaSeq 6000 SP (100 cycles) $2,950.00 $3,687.50
NovaSeq 6000 SP (200 cycles) $3,700.00 $4,625.00
NovaSeq 6000 SP (300 cycles) $4,550.00 $5,687.50
NovaSeq 6000 S1 (100 cycles) $4,800.00 $6,000.00
NovaSeq 6000 S1 (200 cycles) $6,250.00 $7,812.50
NextSeq Hi Output
NextSeq Hi Output (75 cycles) $1,920.00 $2,400.00
NextSeq Hi Output (150 cycles) $3,681.00 $4,601.25
MiSeq v3
MiSeq v3 (600 cycles) $1,988.00 2,485.00
*MiSeq other flowcell info by request


TRADITIONAL SANGER SEQUENCING PRICES

SERVICES PRICES/unit (sample)
  UN System Outside Users
Premixed Sample 
$6 $7.20
Premixed Difficult Sequence Sample $10 $12
Predefined Sample $7 $8.40
Predefined Difficult Sequence Sample $11 $13.20
Custom Sample $10 $12
Custom Difficult Sequence Sample $14 $16.80
Phage Sample $10 $12
Bac Sample $28 $33.60

Difficult Sequence, GC Rich, Hairpin DNA
extra charge per sample

$4 $4.80
Rush Processing per sample additional charge $4 $4.80
Primer Walking Projects ask for estimate
Genotyping Ready-to-Load $9 $10.80
Genotyping Plate Ready-to-Load $150 $180

Notes on Sample Types:

To save money, we recommend that you premix your samples and submit them as Premixed.

Predefined sample is submitted at a higher DNA concentration than required and in a tube separate from the primer.  The primer should be in a separate tube at 5uM; include at least 5uL of primer for each sequencing reaction you request for that primer.  You must measure the sample DNA concentration and provide that information on your order form.  You must include enough DNA and primer to equal or exceed the requirements for the number of reactions you request.  Genewiz will dilute your DNA sample for you, and use the appropriate amount of DNA and primer in their process.

Custom sample is submitted at a higher DNA concentration in a tube separate from the primer. You must include enough DNA and primer to equal or exceed the requirements for the number of sequencing reactions request for them.  The primer must be at a concentration of 5uM or higher.   Thus, a Custom sample is a sample whose DNA concentration you want Genewiz to measure for you and optimize the amount for the sequencing work. 

A difficult sequence sample  is DNA that is GC rich, a hairpin, RNAi sequence or other difficult to sequence DNA.