Prices for various services provided by the UNMC Genomics Core Facility are included for researchers within the University of Nebraska system (UN System), as well as researchers from other academic institutions (Outside Users).
The following prices are effective as of August 1, 2018.
LIBRARY CONSTRUCTION PRICES
| SERVICE | PRICES | |
| UN System | Outside Users | |
| RNA Sequencing Libraries Per Sample | ||
| mRNA Seq (TruSeqV2) | $195.00 | $244.00 |
| miRNA Seq (NextFlexV3) BIOO | $265.00* | $331.25* |
| Total RNA Seq (TruSeq Total Stranded) Library | $235.00 | $293.75 |
| ClontechSmartSeqV4 + Nextera XT | $370.00 | $462.50 |
| *based on a minimum of (3) samples | ||
| DNA Sequencing Libraries Per Sample | ||
| Nextera Flex | $140.00 | $168.00 |
| Metagenomics 16s V3V4 | $15.00 | $18.75 |
NEXT GENERATION SEQUENCING PRICES
| INSTRUMENT | PRICES | ||
| UN System | Outside Users | ||
| NextSeq 500 (price per flowcell) | |||
| NextSeq MidOutput Flowcell V2 150 cycles | $1,387.50 | $1,645.00 | |
| NextSeq MidOutput Flowcell V2 300 cycles | $2,162.50 | $2,575.00 | |
| NextSeq HighOutput Flowcell V2 150 cycles | $3,412.50 | $4,075.00 | |
| NextSeq HighOutput Flowcell V2 300 cycles | $5,400.00 | $6,460.00 | |
| NextSeq HighOutput Flowcell V2 75 cycles | $1,825.00 | $2,170.00 | |
| MiSeqV2 | |||
| MiSeqV2 300 bp paired ends | $1,860.00 | $2,211.40 | |
| MiSeqV2 150 bp paired ends | $1,331.00 | $1,638.75 | |
TRADITIONAL SANGER SEQUENCING PRICES
| SERVICES | PRICES/unit (sample) | ||
| UN System | Outside Users | ||
| Premixed Sample |
$5 | $6 | |
| Premixed Difficult Sequence Sample | $9 | $10.80 | |
| Predefined Sample | $6 | $7.20 | |
| Predefined Difficult Sequence Sample | $10 | $12 | |
| Custom Sample | $9 | $10.80 | |
| Custom Difficult Sequence Sample | $13 | $15.60 | |
| Bulk Sequencing > 94 Samples | $4 | $4.80 | |
| Bulk Sequencing > 94 Difficult Sequence Sample | $8 | $9.60 | |
| Unpurified PCR Reaction Product Sequencing | $8 | $9.60 | |
| Unpurified PCR Reaction Product Difficult Sequence | $12 | $14.40 | |
| Phage Sample | $9 | $10.80 | |
| Bac Sample | $28 | $33.60 | |
|
Difficult Sequence, GC Rich, Hairpin DNA |
$4 | $4.80 | |
| Rush Processing per sample additional charge | $4 | $4.80 | |
| Primer Walking Projects | ask for estimate | ||
| Genotyping Ready-to-Load | $8 | $9.60 | |
| Genotyping Plate Ready-to-Load | $150 | $180 | |
Notes on Sample Types:
To save money, we recommend that you premix your samples and submit them as Premixed.
A Predefined sample is submitted at a higher DNA concentration than required and in a tube separate from the primer. The primer should be in a separate tube at 5uM; include at least 5uL of primer for each sequencing reaction you request for that primer. You must measure the sample DNA concentration and provide that information on your order form. You must include enough DNA and primer to equal or exceed the requirements for the number of reactions you request. Genewiz will dilute your DNA sample for you, and use the appropriate amount of DNA and primer in their process.
A Custom sample is submitted at a higher DNA concentration in a tube separate from the primer. You must include enough DNA and primer to equal or exceed the requirements for the number of sequencing reactions request for them. The primer must be at a concentration of 5uM or higher. Thus, a Custom sample is a sample whose DNA concentration you want Genewiz to measure for you and optimize the amount for the sequencing work.
A difficult sequence sample is DNA that is GC rich, a hairpin, RNAi sequence or other difficult to sequence DNA.