Sperm Cryopreservation

New Service Available

The Mouse Genome Engineering Core Lab now offers sperm cryopreservation and recovery service. We can provide a truly reliable and cost-effective method for preserving and recovering sperm from mouse strains commonly used to make knockout and transgenic mice. This service helps to reduce expenses and facility space requirements associated with maintaining live colonies of strains not under current investigation.
Until recently, available sperm cryopreservation methods have resulted in poor fertilization and have not proven economical for the recovery of live mice. We use the Jackson Laboratory’s new protocol suitable for all commonly used inbred and hybrid genetic backgrounds. For sperm from FVB mice cryopreserved using our service, in vitro fertilization (IVF) rates of 65% or greater are typical.

The protocol requires only two males from your colony.
For a price of $250, we offer cryopreservation with a visual assessment of frozen sperm thawed from one straw. For an additional $250 a quality control check includes an IVF test (to two-cell embryos) to verify fertilization capacity.

Service includes: Recovery Options — Standard Strains

The price for recovery of live mice is $800 in most instances, but does depend on strain background. A typical recovery attempt yields 10 or more mice, though no quantity is guaranteed. Weaned SPF animals are typically available nine to 12 weeks after recovery.


 Dramatically reduces the cost of cryopreserving and recovering mouse strains using fertilized embryos

Cryopreservation Pricing